Molecular linkage mapping in rye (Secale cereale L.)
Molecular linkage mapping in rye (Secale cereale L.)
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DOI：
10.1007/s001220051676
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发表时间：
2001-03-01
影响因子：
5.4
通讯作者：
Gustafson, JP
中科院分区：
文献类型：
--
作者：
Ma, XF;Wanous, MK;Gustafson, JP
黑麦连锁图包含克隆黑麦，小麦，大麦，燕麦和水稻基因组和cDNA文库，已知功能的基因和微卫星标记，使用F-2群体组成的110个F-2衍生的F-3家庭。共显性标记和显性标记都被添加到地图中。在所有筛选的探针中，30.8%是多态性的，其中79.3%被定位。目前的地图包含184个标记存在于所有7个连锁群，覆盖只有727.3 cM。这在整个地图上平均每3.96厘米放置一个标记;然而，仍然存在大的间隙。该地图包含60个标记，已整合从以前的黑麦地图。令人惊讶的是，在1 R短臂中的着丝粒和C1- 1 RS之间没有标记。4号染色体短臂也缺乏足够数量的多态性标记。该群体表现出极显著的偏分离（72.8%）。此外，在黑麦中观察到的遗传距离被发现是非常不同的地图之间创建的不同作图群体。
A rye linkage map containing clones from rye, wheat, barley, oat and rice genomic and cDNA libraries, known-function genes and microsatellite markers, was created using an F-2 population consisting of 110 F-2-derived F-3 families. Both co-dominant and dominant markers were added to the map. Of all probes screened, 30.8% were polymorphic, and of those polymorphic 79.3% were mapped. The current map contains 184 markers present in all seven linkage groups covering only 727.3 cM. This places a marker about every 3.96 cM on average throughout the map; however, large gaps are still present. The map contains 60 markers that have been integrated from previous rye maps. Surprisingly, no markers were placed between the centromere and C1-1RS in the short arm of 1R. The short arm of chromosome 4 also lacked an adequate number of polymorphic markers. The population showed a remarkable degree of segregation distortion (72.8%). In addition, the genetic distance observed in rye was found to be very different among the maps created by different mapping populations.