Influence of PTGS on chalcone synthase gene family in yellow soybean seed coat
Influence of PTGS on chalcone synthase gene family in yellow soybean seed coat
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DOI：
10.1270/jsbbs.54.355
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发表时间：
2004-12-01
期刊：
影响因子：
2.4
通讯作者：
Senda, M
中科院分区：
文献类型：
--
作者：
Kasai, A;Watarai, M;Senda, M
大多数商业大豆品种由于种皮中色素的损失而具有黄色种子。我们以前表明，抑制种皮色素沉着在黄色大豆是由转录后基因沉默（PTGS）的查耳酮合酶（CHS）基因。大豆CHS基因至少由8个成员组成，CHS 1-CHS 8。在这项研究中，我们比较了稳定状态的每个CHS成员的mRNA水平在种皮之间的黄色大豆品种展示CHS PTGS和其色素种皮突变体，其中CHS PTGS被废除。北方杂交分析表明，在有色种皮中，CHS 7和CHS 8的转录本含量较高，而CHS 1-CHS 6的转录本含量较低。逆转录和随后的真实的时间PCR使用成员特异性引物揭示了减少的mRNA的量的大多数CHS成员的黄色大豆的非色素种皮相比，色素种皮的突变体，表明PTGS的CHS基因家族的影响。
Most commercial soybean varieties have yellow seeds due to loss of pigmentation in the seed coat. We previously showed that inhibition of seed coat pigmentation in yellow soybeans is controlled by post-transcriptional gene silencing (PTGS) of chalcone synthase (CHS) genes. Soybean CHS genes are composed of at least eight members, CHS1-CHS8. In this study, we compared the steady state mRNA level of each CHS member in the seed coat between a yellow soybean cultivar displaying CHS PTGS and its pigmented seed coat mutant in which the CHS PTGS is abrogated. Northern hybridization analysis suggested that in the pigmented seed coat, the transcripts of CHS7 and CHS8 were abundant among the total CHS transcripts, whereas those of other CHS members (CHS1-CHS6) were less abundant. Reverse transcription and subsequent real time PCRs using member-specific primers revealed reduction of the amount of mRNA of most CHS members in the non-pigmented seed coat of the yellow soybean as compared to the pigmented seed coat of the mutant, indicating the influence of PTGS on the CHS gene family.