Stress-dependent relocalization of translationally primed mRNPs to cytoplasmic granules that are kinetically and spatially distinct from P-bodies.
Stress-dependent relocalization of translationally primed mRNPs to cytoplasmic granules that are kinetically and spatially distinct from P-bodies.
复制标题
压力依赖性的固定在动力学和空间上与p-bodies不同的细胞质颗粒的重新定位。
DOI：
10.1083/jcb.200707010
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发表时间：
2007-10-08
影响因子：
7.8
通讯作者：
Ashe, Mark P.
中科院分区：
文献类型：
--
作者：
Hoyle, Nathaniel P.;Castelli, Lydia M.;Campbell, Susan G.;Holmes, Leah E. A.;Ashe, Mark P.
胞质RNA颗粒在真核细胞中控制信使RNA（mRNA）命运中起关键作用。例如，在酵母中，严重的应激诱导mRNA重新定位到称为加工体（P体）的降解或储存位点。在这项研究中，我们表明，与葡萄糖饥饿相关的翻译抑制导致mRNA识别的关键翻译介质eIF 4 E、eIF 4G和Pab 1 p从核糖体组分中重新沉积。然后这些介质在P体和以前未识别的胞质体中积累，我们将其定义为EGP体。我们的动力学研究突出了EGP-和P-机构之间的根本区别，并反映了复杂的动力学周围的mRNA库在压力条件下的重新配置。缺乏关键的mRNA衰变因子从EGP机构指向这些机构的mRNA存储功能。总的来说，这项研究突出了新的潜在控制点，在mRNA命运的调节和翻译起始的全球控制。
Cytoplasmic RNA granules serve key functions in the control of messenger RNA (mRNA) fate in eukaryotic cells. For instance, in yeast, severe stress induces mRNA relocalization to sites of degradation or storage called processing bodies (P-bodies). In this study, we show that the translation repression associated with glucose starvation causes the key translational mediators of mRNA recognition, eIF4E, eIF4G, and Pab1p, to resediment away from ribosomal fractions. These mediators then accumulate in P-bodies and in previously unrecognized cytoplasmic bodies, which we define as EGP-bodies. Our kinetic studies highlight the fundamental difference between EGP- and P-bodies and reflect the complex dynamics surrounding reconfiguration of the mRNA pool under stress conditions. An absence of key mRNA decay factors from EGP-bodies points toward an mRNA storage function for these bodies. Overall, this study highlights new potential control points in both the regulation of mRNA fate and the global control of translation initiation.
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影响因子：
4.5
作者：
Brodsky, AS;Silver, PA
通讯作者：
Silver, PA
影响因子：
64.5
作者：
Kiriakidou, Marianthi;Tan, Grace S.;Mourelatos, Zissimos
通讯作者：
Mourelatos, Zissimos
影响因子：
16
作者：
Muckenthaler, M;Gray, NK;Hentze, MW
通讯作者：
Hentze, MW
DOI：
10.1083/jcb.200504039
发表时间：
2005-09-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ferraiuolo MA;Basak S;Dostie J;Murray EL;Schoenberg DR;Sonenberg N
通讯作者：
Sonenberg N
影响因子：
11.4
作者：
Altmann, M;Schmitz, N;Trachsel, H
通讯作者：
Trachsel, H