One-dimensional NOE experiments using pulsed field gradients
One-dimensional NOE experiments using pulsed field gradients
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DOI：
10.1006/jmre.1997.1110
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发表时间：
1997-04-01
影响因子：
2.2
通讯作者：
Shaka, AJ
中科院分区：
文献类型：
--
作者：
Stott, K;Keeler, J;Shaka, AJ
以前，已经表明，在一维NOE实验中使用脉冲保持梯度会产生比以前可能记录的质量高得多的光谱，这种高质量的光谱使得可以完全置信地测量非常小的NOE增强，并且还可以直接测量NOE积累曲线。在本文中，这些NOE实验与梯度背后的自旋动力学进行了详细的分析，强耦合和分子扩散的影响也进行了检查。它还示出了如何可以改进的基本实验，特别是如何不必要的反相贡献（SPT效应）可以完全消除。实验说明的各种方法和改进，并提出实际建议，以如何最好地执行实验。(C)北京：科学出版社.
Previously, it has been shown that the use of pulsed held gradients in one-dimensional NOE experiments results in spectra of much higher quality than it has previously been possible to record, Such high-quality spectra make it possible to measure, with complete confidence, very small NOE enhancements and also make it straight-forward to measure NOE buildup curves. In this paper, the spin dynamics behind these NOE experiments with gradients are analyzed in detail; the effects of strong coupling and molecular diffusion are also examined. It is also shown how the basic experiments can be improved upon, in particular how unwanted antiphase contributions (SPT effects) can be eliminated entirely. Experimental illustrations of the various methods and improvements are given and practical recommendations made as to how the experiments are best performed. (C) 1997 Academic Press.