Effects of free and liposome-encapsulated taxol on two brain tumors xenografted into nude mice.
Effects of free and liposome-encapsulated taxol on two brain tumors xenografted into nude mice.
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游离和脂质体包裹的紫杉醇对裸鼠异种移植的两种脑肿瘤的影响。
作者：
J. Riondel;M. Jacrot;H. Fessi;F. Puisieux;Potier
比较了游离紫杉醇和脂质体包裹的紫杉醇对两个连续移植到雌性无胸腺小鼠肩胛区的人脑肿瘤的抗肿瘤活性。在第一个实验中，研究了人胶质母细胞瘤（第15代和第16代）。在第二个实验中，使用快速生长的人胶质肉瘤（第19代）。游离紫杉醇和紫杉醇脂质体以相同剂量腹腔内给药; 12.5 mg/kg（即评价LD 50值的1/15）。在第一个实验中，治疗连续进行四天，四个疗程之间间隔三天的三个休息期。游离紫杉醇和包封的紫杉醇都产生了统计学上显著的肿瘤生长延迟，并且在实验结束时获得了一些总肿瘤消退。然而，观察到脂质体对胶质母细胞瘤的两次连续传代的作用更有效，使总肿瘤消退的数量显著增加。在第二个实验中，由于异种移植的人神经胶质肉瘤的快速生长模式，选择了另一种治疗方案：连续五天给予游离紫杉醇和脂质体包封的紫杉醇，并进行三个疗程，其中两个休息期为两天。两种形式的紫杉醇对胶质肉瘤肿瘤生长具有显著的抑制作用;如前所述，发现脂质体中的包封增加了紫杉醇的抗肿瘤活性，尽管在这种情况下没有观察到肿瘤消退。
Free taxol and liposome-encapsulated taxol were compared for their antitumoral activities on two human brain tumors serially grafted into female athymic mice in the scapular region. In the first experiment, a human glioblastoma (15th and 16th passages) was studied. In the second experiment, a fast growing human gliosarcoma (19th passage) was used. Free taxol and liposomal taxol were administered intraperitoneally, at the same dose; 12.5 mg/kg (i.e. 1/15 of the evaluated LD 50 value). In the first experiment, the treatment was performed for four consecutive days, with four courses separated by three rest periods of three days in between. Both free taxol and encapsulated taxol produced a statistically significant delay in tumor growth, and at the end of the experiment some total tumor regressions were obtained. However, liposomes were observed to be more effective in their action on the two consecutive passages of the glioblastoma, giving a marked increase of the number of total tumor regressions. In the second experiment another schedule of treatment was chosen because of the fast growth pattern of the xenografted human gliosarcoma: free taxol and liposome-encapsulated taxol were administered for five consecutive days and three courses of treatment were performed with two rest periods of two days. The two forms of taxol had a significant inhibitory effect on gliosarcoma tumor growth; as before encapsulation in liposomes was found to increase the anti-tumoral activity of taxol, although, in this case no tumor regression was observed.