A novel versatile microbiosensor for local hydrogen detection by means of scanning photoelectrochemical microscopy.
A novel versatile microbiosensor for local hydrogen detection by means of scanning photoelectrochemical microscopy.
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DOI：
10.1016/j.bios.2017.03.037
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发表时间：
2017-08
影响因子：
12.6
通讯作者：
Fangyuan Zhao;F. Conzuelo;Volker Hartmann;Huaiguang Li;Stefanie Stapf;M. Nowaczyk;M. Rögner;N. Plumeré;W. Lubitz;W. Schuhmann
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文献类型：
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作者：
Fangyuan Zhao;F. Conzuelo;Volker Hartmann;Huaiguang Li;Stefanie Stapf;M. Nowaczyk;M. Rögner;N. Plumeré;W. Lubitz;W. Schuhmann
报道了一种用于氢气检测的多功能微型生物传感器的研制。将[NiFe]-氢化酶嵌入紫精修饰的氧化还原水凝胶中修饰碳基微电极，制备了灵敏的氢传感器。将该传感器与扫描光电化学显微镜集成，使其既能作为局部光源引发光（生物）电化学反应，又能作为灵敏的氢传感器检测氢。基于嵌入到专门设计的氧化还原聚合物中的光系统1-铂纳米颗粒生物复合物的析氢生物催化剂被用作证明所开发的氢生物传感器在局部照明时检测氢的能力的模型。所提出的微生物传感器作为探针尖端的多功能性和灵敏度允许简化用于评价复杂的电化学过程和快速调查的局部光电催化活性的生物催化剂对光诱导的析氢的设置。
The development of a versatile microbiosensor for hydrogen detection is reported. Carbon-based microelectrodes were modified with a [NiFe]-hydrogenase embedded in a viologen-modified redox hydrogel for the fabrication of a sensitive hydrogen biosensor By integrating the microbiosensor in a scanning photoelectrochemical microscope, it was capable of serving simultaneously as local light source to initiate photo(bio)electrochemical reactions while acting as sensitive biosensor for the detection of hydrogen. A hydrogen evolution biocatalyst based on photosystem 1-platinum nanoparticle biocomplexes embedded into a specifically designed redox polymer was used as a model for proving the capability of the developed hydrogen biosensor for the detection of hydrogen upon localized illumination. The versatility and sensitivity of the proposed microbiosensor as probe tip allows simplification of the set-up used for the evaluation of complex electrochemical processes and the rapid investigation of local photoelectrocatalytic activity of biocatalysts towards light-induced hydrogen evolution.