Isolation and characterization of soluble electron transfer proteins from Chromatium purpuratum.
Isolation and characterization of soluble electron transfer proteins from Chromatium purpuratum.
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从 Chromatium purpuratum 中分离和表征可溶性电子转移蛋白。
DOI：
10.1021/bi952731v
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Knaff,DB
中科院分区：
文献类型：
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作者：
Kerfeld,CA;Chan,C;Hirasawa,M;Kleis-SanFrancisco,S;Yeates,TO;Knaff,DB
从海洋紫色硫细菌Chromatiumpurpuratum中分离并鉴定了几种可溶性电子传递蛋白。C. purpuratumflavocytochromecis在分子量（68 kDa）和等电点（6.5）上与从其它光养生物中分离的flavocytochromes相似。氧化还原滴定的黄细胞色素mechemes显示两个组件的中点电位值为+15和-120 mV，行为类似于所观察到的从嗜热Chromatium tepidum的黄细胞色素。此外，黄素和细胞色素亚基的N-末端氨基酸序列分析表明，基本同源性的一级结构的黄细胞色素的Chromatium vinosum。相反，C.高电位铁硫蛋白（HiPIP）与其他光合细菌分离的蛋白不同之处在于其相对较高的中点电位（+390 mV），以及它在溶液中以二聚体形式存在的可能性。两个低分子质量型细胞色素的特点。一个似乎是高电位（+310 mV）c8型细胞色素。氨基酸序列分析表明，第二个细胞色素可能是一个同源物的低电位cytochromec-551，以前描述的两个物种的外硫罗多菌科。
Several soluble electron transfer proteins were isolated and characterized from the marine purple-sulfur bacteriumChromatiumpurpuratum. TheC. purpuratumflavocytochromecis similar in molecular mass (68 kDa) and isoelectric point (6.5) to flavocytochromes isolated from other phototrophs. Redox titrations of the flavocytochromechemes show two components with midpoint potential values of +15 and −120 mV, behavior similar to that observed with the flavocytochrome isolated from the thermophilicChromatium tepidum.Moreover, N-terminal amino acid sequence analysis of both the flavin and the cytochrome subunit indicates substantial homology to the primary structure of the flavocytochromecofChromatium vinosum. In contrast, theC. purpuratumhigh-potential iron−sulfur protein (HiPIP) differs from those isolated from other photosynthetic bacteria in its relatively high midpoint potential (+390 mV) and the possibility that it exists as a dimer in solution. Two low molecular massc-type cytochromes were also characterized. One appears to be a high-potential (+310 mV)c8-type cytochrome. Amino acid sequencing suggests that the second cytochrome may be a homologue of the low-potential cytochromec-551, previously described in two species of Ectothiorhodospirillaceae.