Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control.
Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control.
复制标题
淋病奈瑟菌中的菌毛蛋白表达受到正转录和负转录控制。
DOI：
10.1002/j.1460-2075.1988.tb03335.x
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Marchal,C
中科院分区：
文献类型：
--
作者：
Taha,MK;So,M;Seifert,HS;Billyard,E;Marchal,C
我们已经确定了两个紧密相连的基因，PilA和PilB，它们作用于Pilin启动子的反式。PILA-PILA-PILB定位于淋球菌染色体中PILA-PILB表达位点PILA-PILA-PILB下游。亚克隆数据表明，PilB对Pilin启动子起负面作用，插入失活的PilB会导致淋球菌毛孔增多。PILA克隆激活了大肠杆菌中的PILA启动子，而PILA-/PILA+异源二倍体淋球菌表现出P-表型。我们无法获得简单的Pila突变体，这有力地表明Pila是淋球菌中的一个必不可少的基因。在体外转录/翻译耦合系统中，跨越PilA和PilB区域的插入片段直接合成了两个40kd和58kd的蛋白质。DNA序列分析表明，PILA和PILB基因分别编码38.6kd和57.9kd的蛋白质(含DNA结合区)。PilA和PilB基因相对方向相反，且两个基因5‘端重叠。我们讨论了这两个基因座如何相互作用来控制淋球菌中菌毛蛋白的表达。
We have identified two closely linked genes, pilA and pilB, which act in trans on the pilin promoter. pilA‐pilB map downstream of expression loci pilE1 and opaE1 in the gonococcal chromosome. Subcloning data indicate that pilB acts negatively on the pilin promoter, and insertional inactivation of pilB results in hyperpiliated gonococci. A pilA clone activates the pilin promoter in Escherichia coli, and a pilA‐/pilA+ heterodiploid gonococcus exhibits a P‐ phenotype. Our inability to obtain simple pilA‐ mutants strongly suggests that pilA is an essential gene in the gonococcus. In an in vitro coupled transcription/translation system, inserts spanning the pilA and pilB region direct the synthesis of two proteins of 40 and 58 kd. DNA sequence analysis shows that the pilA and pilB loci encode proteins of 38.6 kd (with a putative DNA binding domain) and 57.9 kd respectively. The pilA and pilB genes are in opposite orientation relative to each other, and the 5′ ends of the two genes overlap. We discuss how these two loci may interact to control pilin expression in the gonococcus.