THE COMPLETE SEQUENCE OF THE RICE (ORYZA-SATIVA) CHLOROPLAST GENOME - INTERMOLECULAR RECOMBINATION BETWEEN DISTINCT TRANSFER-RNA GENES ACCOUNTS FOR A MAJOR PLASTID DNA INVERSION DURING THE EVOLUTION OF THE CEREALS
THE COMPLETE SEQUENCE OF THE RICE (ORYZA-SATIVA) CHLOROPLAST GENOME - INTERMOLECULAR RECOMBINATION BETWEEN DISTINCT TRANSFER-RNA GENES ACCOUNTS FOR A MAJOR PLASTID DNA INVERSION DURING THE EVOLUTION OF THE CEREALS
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DOI：
10.1007/bf02464880
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发表时间：
1989-06-01
期刊：
影响因子：
--
通讯作者：
SUGIURA, M
中科院分区：
文献类型：
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作者：
HIRATSUKA, J;SHIMADA, H;SUGIURA, M
单子叶水稻（Oryza sativa）的叶绿体基因组全序列已被测定，其长度为134525 bp。预测的基因已被确定沿着与开放阅读框架（ORF）之间的水稻和先前测序的叶绿体基因组，双子叶植物，烟草（烟草），和地钱（多形地钱）。水稻和烟草中有30个tRNA和4个rRNA基因是保守的。大部分ORF在N. tabacum和M.多形体在水稻中也保持完整。然而，几个这样的ORF在水稻中完全不存在，或者仅以严重截短的形式存在。相对于烟草，基因组中的结构变化也很明显。叶绿体基因组结构特征的反向重复序列已经向外扩展，包括烟草和苔类植物中每个基因组仅存在一次的几个基因，并且大的单拷贝区域已经经历了一系列倒位，这些倒位早于谷类植物的分化。一个嵌合tRNA假基因重叠的最大的反转的一个明显的端点，并提出了一个模型调用tRNA基因之间的非法重组，同时占这个假基因的起源，大反转和创建重复序列附近的反转端点。
The entire chloroplast genome of the monocot rice (Oryza sativa) has been sequenced and comprises 134525 bp. Predicted genes have been identified along with open reading frames (ORFs) conserved between rice and the previously sequenced chloroplast genomes, a dicot, tobacco (Nicotiana tabacum), and a liverwort (Marchantia polymorpha). The same complement of 30 tRNA and 4 rRNA genes has been conserved between rice and tobacco. Most ORFs extensively conserved between N. tabacum and M. polymorpha are also conserved intact in rice. However, several such ORFs are entirely absent in rice, or present only in severely truncated form. Structural changes are also apparent in the genome relative to tobacco. The inverted repeats, characteristic of chloroplast genome structure, have expanded outward to include several genes present only once per genome in tobacco and liverwort and the large single copy region has undergone a series of inversions which predate the divergence of the cereals. A chimeric tRNA pseudogene overlaps an apparent endpoint of the largest inversion, and a model invoking illegitimate recombination between tRNA genes is proposed which accounts simultaneously for the origin of this pseudogene, the large inversion and the creation of repeated sequences near the inversion endpoints.