Determination of beta-Blockers in Bovine and Porcine Tissues and Bovine Milk by High-Performance Liquid Chromatography - Tandem Mass Spectrometry
Determination of beta-Blockers in Bovine and Porcine Tissues and Bovine Milk by High-Performance Liquid Chromatography - Tandem Mass Spectrometry
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采用高效液相色谱-串联质谱法测定牛、猪组织和牛乳中的 β 受体阻滞剂
DOI：
10.1080/00032719.2018.1470638
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发表时间：
2019
影响因子：
2
通讯作者：
Wang Zhi Qiang
中科院分区：
文献类型：
--
作者：
Xiao Xia;Zhang Min Min;Wang Zhi Qiang
在食用动物中非法使用β-阻滞剂可能会导致肉和奶中出现这些化合物。这些食品中β受体阻滞剂的存在是一个安全问题。建立了一种简单、经济的高效液相色谱-串联质谱分析方法，并对牛和猪肌肉、肾脏、肝脏和牛奶中的β受体阻滞剂进行了验证。本研究的重点是对乙酰布洛尔、阿替洛尔、倍他洛尔、卡拉唑尔、美托洛尔、纳多洛尔、喷布洛尔和心得安的检测和定量。匀浆后的组织用葡萄糖醛酸酶/芳基硫酸酯酶消化，释放分析物，乙腈提取，基质固相分散纯化。对于牛奶中的残留物，采用三氯乙酸和乙腈进行酸解和萃取，采用混合模式阳离子交换固相萃取对样品进行纯化。均质组织与乳基质形成的标准曲线呈线性关系，相关系数大于0.99。肉组织中所有分析物的检出限和定量限分别为1 μg/kg和2.5 μg/kg。牛奶的相应值分别为0.2和0.5 μg/kg。加标样品的平均加样回收率为84.4 ~ 114.2%，日内和日间测定的标准偏差分别为2.0 ~ 14.6%和2.9 ~ 18.7%。本方法简便、经济、省时，适用于牛组织、猪组织和牛乳中β-阻滞剂的测定。
The illicit use of β-blockers in food-producing animals may induce the presence of these compounds in meat and milk. The presence of β-blockers in these foods is a safety issue. A simple and economic high-performance liquid chromatography – tandem mass spectrometry method was developed and validated for β-blockers in bovine and porcine muscle, kidney, liver, and bovine milk. The focus of the study was on the detection and quantitation of acebutolol, atenolol, betaxolol, carazolol, metoprolol, nadolol, penbutolol, and propranolol. Homogenized tissues were digested with glucuronidase/aryl sulfatase to release the analytes that were extracted with acetonitrile and purified using matrix solid-phase dispersion. For residues in milk, acidolysis and extraction utilized trichloroacetic acid and acetonitrile and the samples were purified using mixed-mode cation exchange solid phase extraction. Standard curves generated using homogenized tissues and milk matrices were linear with correlation coefficients exceeding 0.99. The limits of detection and quantification were 1 μg/kg and 2.5 μg/kg, respectively, for all analytes in the meat tissues. The corresponding values for milk were 0.2 μg/kg and 0.5 μg/kg. The average recoveries of the spiked samples were from 84.4 to 114.2% with the standard deviations of the intra- and inter-day assays from 2.0 to 14.6% and 2.9 to 18.7%, respectively. This method is simple, economical, and time-saving for the determination of β-blockers in bovine tissue, porcine tissue, and bovine milk.