Systematic analysis of the relation of electron transport and ATP synthesis to the photodamage and repair of photosystem II in Synechocystis
Systematic analysis of the relation of electron transport and ATP synthesis to the photodamage and repair of photosystem II in Synechocystis
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DOI：
10.1104/pp.104.054478
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发表时间：
2005-01-01
期刊：
影响因子：
7.4
通讯作者：
Murata, N
中科院分区：
文献类型：
--
作者：
Allakhverdiev, SI;Nishiyama, Y;Murata, N
光合机制，特别是光系统11（PSII）复合体对强光敏感，强光的影响被称为光损伤或光抑制。在生物体中，光损伤的PSII被迅速修复，因此，光抑制的程度代表了光损伤率和PSII修复率之间的平衡。在这项研究中，我们研究了电子传递和ATP合成在这两个过程中的作用，分别和系统地监测它们在蓝藻集胞藻属PCC 6803。我们发现，光损伤的速率，这是成正比的光强度，是不受抑制的电子传递在PSII，加速电子传递在PSI，和抑制ATP的合成。相比之下，无论是通过PSI或PSII的ATP的合成抑制后，修复率降低。北方印迹和[S-35]Met放射性标记分析表明，ATP的合成促进了D1蛋白的合成。我们的观察结果表明，ATP的合成可能会调节PSII的修复，特别是在水平上的翻译的psbA基因的前体D1蛋白，而电子传递也不ATP的合成影响光损伤的程度。
The photosynthetic machinery and, in particular, the photosystem 11 (PSII) complex are susceptible to strong light, and the effects of strong light are referred to as photodamage or photoinhibition. In living organisms, photodamaged PSII is rapidly repaired and, as a result, the extent of photoinhibition represents a balance between rates of photodamage and the repair of PSII. In this study, we examined the roles of electron transport and ATP synthesis in these two processes by monitoring them separately and systematically in the cyanobacterium Synechocystis sp. PCC 6803. We found that the rate of photodamage, which was proportional to light intensity, was unaffected by inhibition of the electron transport in PSII, by acceleration of electron transport in PSI, and by inhibition of ATP synthesis. By contrast, the rate of repair was reduced upon inhibition of the synthesis of ATP either via PSI or PSII. Northern blotting and radiolabeling analysis with [S-35]Met revealed that synthesis of the D1 protein was enhanced by the synthesis of ATP. Our observations suggest that ATP synthesis might regulate the repair of PSII, in particular, at the level of translation of the psbA genes for the precursor to the D1 protein, whereas neither electron transport nor the synthesis of ATP affects the extent of photodamage.