Advanced Methods for High-Throughput Microscopy Screening of Genetically Modified Yeast Libraries
Advanced Methods for High-Throughput Microscopy Screening of Genetically Modified Yeast Libraries
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DOI：
10.1007/978-1-61779-276-2_8
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Schuldiner, Maya
中科院分区：
文献类型：
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作者：
Cohen, Yifat;Schuldiner, Maya
高通量方法为以公正的方式研究生物现象创造了新的机会。使用自动化细胞操作和显微镜平台，现在可以轻松地筛选整个基因组中影响任何可以可视化的细胞过程的基因。这些方法的出现预示着在不久的将来，将带来对复杂和动态的细胞结构和过程的更全面和更丰富的综合理解。在这篇综述中，我们描述了如何在芽殖酵母酿酒酵母（Saccharomyces cerevisiae）中结合系统遗传工具与机器人可视化系统来解决生物学问题。高通量显微镜屏幕与强大而简单的酵母模型系统相结合，用于研究真核细胞，应该在细胞生物学的所有领域开拓新的知识。
High-throughput methodologies have created new opportunities for studying biological phenomena in an unbiased manner. Using automated cell manipulations and microscopy platforms, it is now possible to easily screen entire genomes for genes that affect any cellular process that can be visualized. The onset of these methodologies promises that the near future will bring with it a more comprehensive and richly integrated understanding of complex and dynamic cellular structures and processes. In this review, we describe how to couple systematic genetic tools in the budding yeast Saccharomyces cerevisiae alongside robotic visualization systems to attack biological questions. The combination of high-throughput microscopy screens with the powerful, yet simple, yeast model system for studying the eukaryotic cell should pioneer new knowledge in all areas of cell biology.