Measuring persistent temporomandibular joint nociception in rats and two mice strains
Measuring persistent temporomandibular joint nociception in rats and two mice strains
复制标题
DOI：
10.1016/j.physbeh.2010.01.037
复制
发表时间：
2010-04-19
影响因子：
2.9
通讯作者：
Bellinger, Larry L.
中科院分区：
文献类型：
--
作者：
Kramer, Phillip R.;Kerins, Carolyn A.;Bellinger, Larry L.
据报道，人类颞下颌关节 (TMJ) 疼痛会持续很长时间。在啮齿类动物中，有多种方法可用于测量颞下颌关节疼痛，但大多数方法的测量时间为几分钟到几个小时。此外，大多数测量方案都需要对动物进行约束或训练。我们实验室之前的研究表明，进食行为，特别是进餐时间，是不受限制和未经训练的雄性和雌性 Sprague-Dawley 大鼠长达两天的颞下颌关节伤害感受的指标。在这项研究中，我们首先发现，向大鼠颞下颌关节注射完全弗氏佐剂（CFA）可显着延长进餐时间19天，并减少进餐频率42天。有趣的是，在 19 天的时间里，每天的进餐时间都有很大差异。在注射 CFA 的动物的 TMJ 组织中，TMJ 白细胞介素 1 β (IL-1 β) 和降钙素基因相关肽 (CGRP) 显着升高，并且这些标记物的水平随着进餐时间的延长而减弱。将盐水注射到TMJ或将CFA注射到膝盖的对照动物没有表现出进餐时间的显着延长，但确实表现出进餐频率的减少。在第二项研究中，注射 TMJ CFA 的 DBA/1LacJ 小鼠在使用 5 或 10 分钟进餐结束定义测量的 7 天中的 4 天中表现出进餐时间显着延长。其他膳食模式没有显着变化。 CFA 注射两天后，DBA/1LacJ 小鼠表现出白细胞介素 6 (IL-6) 显着升高，但 IL-1 β 未升高。注射后 7 天，IL-6 和 IL-1 β 均显着升高。未检测到 CGRP 变化。在这项研究中，C57Bl/6 小鼠也接受了 TMJ CFA 注射，但它们没有表现出任何进餐模式的延长或 IL-1β、IL-6 或 CGRP 的显着增加。我们的数据首次表明，进餐时间可用于测量 CFA 在不受约束的大鼠中数周内引起的 TMJ 伤害感受，以及在 DBA/1LacJ 小鼠品系中长达 7 天的时间。此外，在与 DBA/1LacJ 小鼠相同的剂量下，C57Bl/6 小鼠对 CFA 诱导的 TMJ 伤害感受具有抵抗力。 (C) 2010 Elsevier Inc. 保留所有权利。
Temporomandibular joint (TMJ) pain has been reported to last for prolonged periods in humans. In rodents a variety of methods have been used to measure TMJ nociception, but for most of these methods the period of measurement has been minutes to a couple of hours. In addition, most measurement protocols required restraint or training of the animal. Previous studies from our laboratory demonstrated that feeding behavior, particularly meal duration, was an indicator of TMJ nociception in unrestrained and untrained male and female Sprague-Dawley rats for up to two days. In this study, we first found that injection of complete Freund's adjuvant (CFA) into the TMJ of rats significantly lengthened meal duration for 19 days and also decreased meal frequency for 42 days. Interestingly, the meal duration varied significantly from day to day within the 19 day period. TMJ interleukin-1 beta (IL-1 beta) and calcitonin gene-related peptide (CGRP) were significantly elevated in the TMJ tissues of CFA-injected animals and the level of these markers was attenuated as the meal duration decreased with time. Control animals injected with saline into the TMJ or CFA into the knee did not show a significant lengthening in meal duration but did show a decrease in meal frequency. In a second study, DBA/1LacJ mice given TMJ CFA injections showed a significantly lengthened meal duration on four of the seven days measured using end-of-the meal definition of 5 or 10 min. No other meal pattern changed significantly. Two days post-CFA injection, the DBA/1LacJ mice showed significantly elevated interleukin-6 (IL-6), but not elevated IL-1 beta, Seven days post-injection, both IL-6 and IL-1 beta, were significantly elevated. No change in CGRP was detected. In this study C57Bl/6 mice also received TMJ CFA injections, but they did not show a lengthening in any meal pattern or significant increases in IL-1 beta, IL-6 or CGRP. Our data show, for the first time, that meal duration can be used to measure CFA-induced nociception in the TMJ over the course of several weeks in unrestrained rats and for up to seven days in the DBA/1LacJ mouse strain. In addition, C57Bl/6 mice are resistant to CFA-induced TMJ nociception at the same dose used in the DBA/1LacJ mice. (C) 2010 Elsevier Inc. All rights reserved.