Biofuel components change the ecology of bacterial volatile petroleum hydrocarbon degradation in aerobic sandy soil.
Biofuel components change the ecology of bacterial volatile petroleum hydrocarbon degradation in aerobic sandy soil.
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DOI：
10.1016/j.envpol.2012.10.010
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发表时间：
2013-02
影响因子：
8.9
通讯作者：
Abdulmagid Elazhari-Ali;A. Singh;R. Davenport;I. Head;D. Werner
中科院分区：
文献类型：
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作者：
Abdulmagid Elazhari-Ali;A. Singh;R. Davenport;I. Head;D. Werner
我们测试了一个假设，即挥发性石油烃（VPHs）在好氧桑迪土壤中的生物降解的影响与10%的乙醇（E10）或20%的生物柴油（B20）的混合。当无机营养物缺乏时，生物燃料和VPH降解剂之间的竞争暂时减缓了单芳烃的降解。乙醇比生物柴油的影响更大，这反映了乙醇比甲酯生物降解相对容易。细菌16 S rRNA基因的变性梯度凝胶电泳（DGGE）显示，每种燃料混合物选择了一个不同的细菌群落，每一个占主导地位的假单胞菌属。尽管对土壤细菌生态学有持久的影响，但对VHP生物降解的总体影响很小，燃料类型之间的平均生物量产量相当，范围为0.40 ± 0.16至0.51 ± 0.22克生物量碳/克燃料碳降解。无机养分的可利用性对石油烃生物降解的影响大于燃料成分。
We tested the hypothesis that the biodegradation of volatile petroleum hydrocarbons (VPHs) in aerobic sandy soil is affected by the blending with 10 percent ethanol (E10) or 20 percent biodiesel (B20). When inorganic nutrients were scarce, competition between biofuel and VPH degraders temporarily slowed monoaromatic hydrocarbon degradation. Ethanol had a bigger impact than biodiesel, reflecting the relative ease of ethanol compared to methyl ester biodegradation. Denaturing gradient gel electrophoresis (DGGE) of bacterial 16S rRNA genes revealed that each fuel mixture selected for a distinct bacterial community, each dominated by Pseudomonas spp. Despite lasting impacts on soil bacterial ecology, the overall effects on VHP biodegradation were minor, and average biomass yields were comparable between fuel types, ranging from 0.40 ± 0.16 to 0.51 ± 0.22 g of biomass carbon per gram of fuel carbon degraded. Inorganic nutrient availability had a greater impact on petroleum hydrocarbon biodegradation than fuel composition.