Tests of the chromatographic theory of olfaction with highly soluble odors: a combined electro-olfactogram and computational fluid dynamics study in the mouse
Tests of the chromatographic theory of olfaction with highly soluble odors: a combined electro-olfactogram and computational fluid dynamics study in the mouse
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DOI：
10.1242/bio.047217
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发表时间：
2019-10-01
期刊：
影响因子：
2.4
通讯作者：
Craven, Brent A.
中科院分区：
文献类型：
--
作者：
Coppola, David M.;Fitzwater, Emily;Craven, Brent A.
脊椎动物的鼻腔就像一台气相色谱仪，用来分离和辨别气味，这一观点在这里被称为“色谱理论”(CT)，这一想法有着悠久而有趣的历史。尽管在过去的十年里，人们对这一概念重新产生了兴趣，但它的有效性仍然存在疑问。在这里，我们检验了该理论的一个必要条件：基于粘液溶解度的鼻臭沉积模式与嗅觉感觉神经元分布之间的相关性。我们最近在小鼠身上的工作未能发现这种关系，即使在嗅觉上皮(OE)内的大吸收梯度上也是如此。然而，这些研究没有测试极易溶解的气味或低气味浓度，这些因素可以解释我们无法为CT找到支持证据。目前的研究结合了气味吸收模式的计算流体动力学(CFD)模拟和嗅觉神经元反应的电嗅觉图(EOG)测量。被测试的气味处于粘液溶解度的极端和浓度的范围内。结果表明，局部气味吸收模式与EOG反应图之间没有关系。总而言之，结果再次未能支持CT的必要条件，进一步怀疑这一经典气味编码机制的可行性。
The idea that the vertebrate nasal cavity operates like a gas chromatograph to separate and discriminate odors, referred to herein as the 'chromatographic theory' (CT), has a long and interesting history. Though the last decade has seen renewed interest in the notion, its validity remains in question. Here we examine a necessary condition of the theory: a correlation between nasal odor deposition patterns based on mucus solubility and the distribution of olfactory sensory neuron odotypes. Our recent work in the mouse failed to find such a relationship even across large sorption gradients within the olfactory epithelium (OE). However, these studies did not test extremely soluble odorants or low odor concentrations, factors that could explain our inability to find supporting evidence for the CT. The current study combined computational fluid dynamics (CFD) simulations of odor sorption patterns and electro-olfactogram (EOG) measurements of olfactory sensory neuron responses. The odorants tested were at the extremes of mucus solubility and at a range of concentrations. Results showed no relationship between local odor sorption patterns and EOG response maps. Together, results again failed to support a necessary condition of the CT casting further doubt on the viability of this classical odor coding mechanism.