Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, lamas, and rabbits
Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, lamas, and rabbits
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DOI：
10.1016/j.vetimm.2007.06.024
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发表时间：
2007-09-15
影响因子：
1.8
通讯作者：
Hamilton, Mary Jo
中科院分区：
文献类型：
--
作者：
Davis, William C.;Drbal, Karel;Hamilton, Mary Jo
使用流式细胞术筛选提交给HLDA 8动物同源物部分的一组320个mAb，以识别山羊、喇嘛和兔中直链白细胞分化抗原（LDA）上保守的表位。19种对CD 11 a（1）、CD 14（3）、CD 18（1）、CD 21（1）、CD 29（2）、CD 44（2）、CD 47（3）、CD 49 d（1）、CD 172 a（1）、CD 45 RB（1）、CD 61（1）、RACT 48 A和GBSP 71 A具有特异性的mAb与山羊LDA反应。对CD7（1）、CD9（2）、CD11a（1）、CD14（3）、CD18（4）、CD29（1）、CD32（1）、CD44（1）、CD47（4）、CD49d（2）、CD50（1）、CD80（1）、CD172a（1）和GBSP71A特异的23种mAb与美洲驼LDA反应。对CD9（2）、CD11a（1）、CD14（2）、CD18（4）、CD21（1）、CD44（2）、CD45RB（1）、CD49d（1）、CD209（1）、RACT48A和GBSP71A具有特异性的18种mAb与兔LDA反应。尚未确定识别两个物种（RACT 48 A）和所有三个物种（GBSP 71 A）中所有白细胞上不同保守表位的两种交叉反应性mAb的特异性。大多数mAb的反应性模式与人白细胞上观察到的反应性模式一致。在人白细胞上验证了在非人种属中生成的一些交叉反应性mAb的特异性。需要进一步的研究来验证CD7、CD32、CD45RB、CD50和CD209识别指定物种中的邻位配体分子。(c)2007 Elsevier B.V.保留所有权利。
Flow cytometry was used to screen a panel of 320 mAbs, submitted to the Animal Homologues Section of the HLDA8, for mAbs that recognize epitopes conserved on orthologous leukocyte differentiation antigens (LDA) in goats, lamas, and rabbits. Nineteen mAbs specific for CD11a (1), CD14 (3), CD 18 (1), CD21 (1), CD29 (2), CD44 (2), CD47 (3), CD49d (1), CD172a (1), CD45RB (1), CD61 (1), RACT48A, and GBSP71 A reacted with goat LDA. Twenty three mAbs specific for CD7 (1), CD9 (2), CD I I a (1), CD 14 (3), CD 18 (4), CD29 (1), CD32 (1), CD44 (1), CD47 (4), CD49d (2), CD50 (1), CD80 (1), CD172a (1), and GBSP71A reacted with llama LDA. Eighteen mAbs specific for CD9 (2), CD I I a (1), CD 14 (2), CD 18 (4), CD21 (1), CD44 (2), CD45RB (1), CD49d (1), CD209 (1), RACT48A, and GBSP71 A reacted with rabbit LDA. The specificities of two cross reactive mAbs that recognize different conserved epitopes on all leukocytes in two species (RACT48A) and all three species (GBSP71A) have not been determined. The patterns of reactivity of most of the mAbs were consistent with patterns of reactivity noted on human leukocytes. The specificity of some cross reactive mAbs generated in non-human species were validated on human leukocytes. Further studies are needed to verify that CD7, CD32, CD45RB, CD50, and CD209 recognize orthologous molecules in the indicated species. (c) 2007 Elsevier B.V. All rights reserved.