Recent progress in the molecular imaging of therapeutic monoclonal antibodies.
Recent progress in the molecular imaging of therapeutic monoclonal antibodies.
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治疗性单克隆抗体分子影像的最新进展
DOI：
10.1016/j.jpha.2020.07.006
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发表时间：
2020-10
影响因子：
8.8
通讯作者：
Qian L
中科院分区：
文献类型：
--
作者：
He K;Zeng S;Qian L
治疗性单克隆抗体已成为医疗保健系统的核心组成部分之一，并且不断努力为有需要的患者带来创新的抗体疗法。由于个体差异和耐药性等新挑战，充分了解其分子结构和生物学功能，通过结合新的检测方法来确保有效性和安全性也同样重要。用于确定抗体分布的传统技术，包括使用 LC-MS 或 ELISA 测量血浆药物浓度，以及使用免疫组织化学和免疫荧光测量组织分布，现在辅以分子成像方式，如正电子发射断层扫描和近红外荧光成像，以获得更多动态信息，而表征抗体与靶抗原相互作用以及其细胞和细胞间行为可视化的方法仍在开发中。本文描述了检测治疗性抗体的最新进展，特别是适合说明分子动力学的方法的开发。治疗性单克隆抗体的检测方法，以更好地了解其功效和安全性。分子成像模式适合监测抗体治疗的体内到亚细胞动力学。可激活荧光探针，用于抗体及其与活细胞相互作用的免洗实时成像。
Therapeutic monoclonal antibodies have become one of the central components of the healthcare system and continuous efforts are made to bring innovative antibody therapeutics to patients in need. It is equally critical to acquire sufficient knowledge of their molecular structure and biological functions to ensure the efficacy and safety by incorporating new detection approaches since new challenges like individual differences and resistance are presented. Conventional techniques for determining antibody disposition including plasma drug concentration measurements using LC-MS or ELISA, and tissue distribution using immunohistochemistry and immunofluorescence are now complemented with molecular imaging modalities like positron emission tomography and near-infrared fluorescence imaging to obtain more dynamic information, while methods for characterization of antibody’s interaction with the target antigen as well as visualization of its cellular and intercellular behavior are still under development. Recent progress in detecting therapeutic antibodies, in particular, the development of methods suitable for illustrating the molecular dynamics, is described here. Detection methods for therapeutic monoclonal antibodies to better understand their efficacy and safety. Molecular imaging modalities suitable for monitoring the in vivo to subcellular dynamics of antibody therapeutics. Activatable fluorescent probes for no-wash real-time imaging of antibody and its interaction with living cells.
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影响因子：
3.1
作者：
Adumeau P;Sharma SK;Brent C;Zeglis BM
通讯作者：
Zeglis BM
影响因子：
8.8
作者：
Carillo, Sara;Perez-Robles, Raquel;Bones, Jonathan
通讯作者：
Bones, Jonathan
影响因子：
4.6
作者：
Beeg, Marten;Nobili, Alessandro;Gobbi, Marco
通讯作者：
Gobbi, Marco
大小依赖性分离控制巨噬细胞对抗体调理靶标的吞噬作用。
影响因子：
64.5
作者：
Bakalar MH;Joffe AM;Schmid EM;Son S;Podolski M;Fletcher DA
通讯作者：
Fletcher DA
影响因子：
--
作者：
Bernhard W;El-Sayed A;Barreto K;Gonzalez C;Hill W;Parada AC;Fonge H;Geyer CR
通讯作者：
Geyer CR