Detection of Uric Acid in the Presence of Dopamine and High Concentration of Ascorbic Acid Using PDDA Modified Graphite Electrode
Detection of Uric Acid in the Presence of Dopamine and High Concentration of Ascorbic Acid Using PDDA Modified Graphite Electrode
复制标题
DOI：
10.1002/elan.200904662
复制
发表时间：
2009-10
期刊：
影响因子：
3
通讯作者：
H. Manjunatha;D. H. Nagaraju;G. Suresh;T. V. Venkatesha
中科院分区：
文献类型：
--
作者：
H. Manjunatha;D. H. Nagaraju;G. Suresh;T. V. Venkatesha
用循环伏安法、差示脉冲伏安法和计时电流法研究了聚二烯丙基二甲基氯化铵(PDDA)修饰的石墨电极在多巴胺(DA)和高浓度抗坏血酸(AA)存在下对尿酸(UA)的检测性能。用一种非常简单的方法制备了聚合物修饰石墨电极，只需将石墨电极在PDDA溶液中浸泡20分钟。与裸石墨电极相比，PdDA/Gr修饰电极对UA、DA和AA的氧化表现出良好的电催化活性。在循环伏安法上，UA、DA和AA的电化学峰电位分别为220 mV、168 mV和387 mV；在差示脉冲伏安法中，相应的峰电位分别为230 mV、130 mV和354 mV。对UA、DA和AA的最低检出限分别为1×10−7M、2×10−7M和80 0×10−9M。PDDA/Gr电极具有良好的选择性、灵敏度和重现性，有效地消除了DA和高浓度AA对UA测定的干扰。该修饰电极还成功地用于三元混合物中UA、DA和AA的同时测定。
The properties of graphite electrode (Gr) modified with poly(diallyl dimethyl ammonium chloride) (PDDA) for the detection of uric acid (UA) in the presence of dopamine (DA) and high concentration of ascorbic acid (AA) have been investigated by cyclic voltammetry, differential pulse voltammetry and chronoamperometry. The polymer modified graphite electrode was prepared by a very simple method just by immersing the graphite electrode in PDDA solution for 20 minutes. The PDDA/Gr modified electrode displayed excellent electrocatalytic activity towards the oxidation of UA, DA and AA compared to that at the bare graphite electrode. The electrochemical oxidation signals of UA, DA and AA are well resolved into three distinct peaks with peak potential separations of 220 mV, 168 mV and 387 mV between AA-DA, DA-UA and AA-UA respectively in cyclic voltammetry studies and the corresponding peak potential separations are 230 mV, 130 mV and 354 mV respectively in differential pulse voltammetry. The lowest detection limits obtained for UA, DA and AA were 1×10−7 M, 2×10−7 M and 800×10−9 M respectively. The PDDA/Gr electrode efficiently eliminated the interference of DA and a high concentration of AA in the determination of UA with good selectivity, sensitivity and reproducibility. The modified electrode was also successfully applied for simultaneous determination of UA, DA and AA in their ternary mixture.