Scaffolds from block polyurethanes based on poly(ε-caprolactone) (PCL) and poly(ethylene glycol) (PEG) for peripheral nerve regeneration
Scaffolds from block polyurethanes based on poly(ε-caprolactone) (PCL) and poly(ethylene glycol) (PEG) for peripheral nerve regeneration
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DOI：
10.1016/j.biomaterials.2014.02.013
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发表时间：
2014-05-01
期刊：
影响因子：
14
通讯作者：
Xu, Kaitian
中科院分区：
文献类型：
--
作者：
Niu, Yuqing;Chen, Kevin C.;Xu, Kaitian
采用颗粒浸出法制备了不含任何生长因子或蛋白质的块聚氨酯神经导向支架。基于聚己内酯（pcl -二醇）和聚乙二醇（PEG）的嵌段聚氨酯（简称PUCL-ran-EG）支架具有高表面积的多孔性，可用于细胞附着，并可提供生化和地形线索以促进组织再生。神经导向支架孔径1 ~ 5 μ m，孔隙率88%。力学试验表明，该聚氨酯神经导支架的最大载荷为4.98 +/- 0.35 N，最大应力为6372 +/- 0.5 MPa。在大鼠周围神经损伤模型中测试了这些神经引导支架的组织相容性。在大鼠模型中比较了PUCL-ran-EG支架、自体移植物、PCL支架和硅胶管四种类型的支架。14周后，所有大鼠均观察到再生神经对10 mm缺损间隙的桥接。采用坐骨功能指数（SFI）、HE染色、免疫组化、氨银染色、马松三色染色及透射电镜观察等组织学方法对神经再生进行系统表征。结果表明，聚氨酯神经引导支架的再生性能明显优于PCL、硅胶管组，可与自体移植物媲美。PCL组、PUCL-ran-EG组和自体移植物组的电生理恢复率分别为36%、76%和87%，硅胶管组的电生理恢复率为29.8%。体外和体内生物降解实验表明，pcl -ran- eg神经引导支架具有良好的降解能力。本研究表明，无需进一步修饰，plain PUCL-ran-EG神经引导支架可以很好地帮助周围神经再生。(C) 2014 Elsevier Ltd.版权所有。
Nerve guide scaffolds from block polyurethanes without any additional growth factors or protein were prepared using a particle leaching method. The scaffolds of block polyurethanes (abbreviated as PUCL-ran-EG) based on poly(epsilon-caprolactone) (PCL-diol) and poly(ethylene glycol) (PEG) possess highly surface-area porous for cell attachment, and can provide biochemical and topographic cues to enhance tissue regeneration. The nerve guide scaffolds have pore size 1-5 mu m and porosity 88%. Mechanical tests showed that the polyurethane nerve guide scaffolds have maximum loads of 4.98 +/- 0.35 N and maximum stresses of 6372 +/- 0.5 MPa. The histocompatibility efficacy of these nerve guide scaffolds was tested in a rat model for peripheral nerve injury treatment. Four types of guides including PUCL-ran-EG scaffolds, autograft, PCL scaffolds and silicone tubes were compared in the rat model. After 14 weeks, bridging of a 10 mm defect gap by the regenerated nerve was observed in all rats. The nerve regeneration was systematically characterized by sciatic function index (SFI), histological assessment including HE staining, immunohistochemistry, ammonia silver staining, Masson's trichrome staining and TEM observation. Results revealed that polyurethane nerve guide scaffolds exhibit much better regeneration behavior than PCL, silicone tube groups and comparable to autograft. Electrophysiological recovery was also seen in 36%, 76%, and 87% of rats in the PCL, PUCL-ran-EG, and autograft groups respectively, whilst 29.8% was observed in the silicone tube groups. Biodegradation in vitro and in vivo show proper degradation of the PUCL-ran-EG nerve guide scaffolds. This study has demonstrated that without further modification, plain PUCL-ran-EG nerve guide scaffolds can help peripheral nerve regeneration excellently. (C) 2014 Elsevier Ltd. All rights reserved.