Food supplementation with milk fermented by Lactobacillus casei DN-114 001 protects suckling rats from rotavirus-associated diarrhea
Food supplementation with milk fermented by Lactobacillus casei DN-114 001 protects suckling rats from rotavirus-associated diarrhea
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DOI：
10.1093/jn/131.1.111
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发表时间：
2001-01-01
影响因子：
4.2
通讯作者：
Andrieux, C
中科院分区：
文献类型：
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作者：
Guérin-Danan, C;Meslin, JC;Andrieux, C
A组轮状病毒是全球3-36个月儿童腹泻的主要原因。已提出将发酵乳产品引入婴儿饮食以预防或治疗轮状病毒腹泻。在无菌乳鼠模型中研究了干酪乳杆菌菌株DN-114 001发酵的牛奶的预防效果，该模型从出生后第2天开始每天补充无菌乳鼠，并在第5天感染SA 11轮状病毒（RF组）。一组补充非发酵奶（RM）和两个未感染组（CM和CF）接受非发酵或发酵奶。观察腹泻的频率和严重程度。在感染后0至120 h（p.i.）的不同时间处死大鼠。在肠道中测量细菌，并通过ELISA检测粪便样品和肠道不同部位中的轮状病毒抗原。进行组织学观察，包括空泡化、肠绒毛形态和粘蛋白细胞数量。RM大鼠腹泻6 d;与CM组相比，它们在p.i. 48和72 h出现以细胞空泡化为特征的肠粘膜改变。和较低数量的硫酸化粘蛋白细胞72和96 h p.i.（P < 0.05）。早期补充发酵乳可显著降低感染后24至144 h的腹泻临床体征。（P < 0.05），并阻止了各肠段的轮状病毒感染。小肠组织学病变明显减轻（P < 0.05），粘蛋白细胞数无明显变化。这些数据进行了讨论，以减少轮状病毒腹泻的幼儿消费发酵奶的可能性。
Group A rotavirus is the leading cause of diarrhea among children aged 3-36 mo worldwide. Introducing fermented milk products into the infant diet has been proposed for the prevention or treatment of rotavirus diarrhea. The preventive effect of milk fermented by the Lactobacillus casei strain DN-114 001 was studied in a model of germfree suckling rats supplemented daily from d 2 of life and infected with SA11 rotavirus at d 5 (RF group). One group was supplemented with nonfermented milk (RM) and two uninfected groups (CM and CF) received either nonfermented or fermented milk. Frequency and severity of diarrhea were observed. Rats were killed at various times from 0 to 120 h postinfection (p.i.). Bacteria were measured in the intestine, and rotavirus antigens were detected by ELISA in fecal samples and in different parts of the intestine. Histologic observations were made, including vacuolation, morphology of intestinal villi and number of mucin cells. RM rats had diarrhea for 6 d; compared with the CM group, they had alterations of the intestinal mucosa characterized by cellular vacuolation 48 and 72 h p.i. and a lower number of sulfated mucin cells 72 and 96 h p.i. (P < 0.05). Early supplementation with fermented milk significantly decreased the clinical signs of diarrhea from 24 to 144 h p.i. (P < 0.05) and prevented rotavirus infection in all sections of the intestine. Histologic lesions of the small intestine were greatly reduced (P < 0.05) and the number of mucin cells remained unchanged. The data are discussed with respect to the possibility of reducing rotavirus diarrhea in young children by consumption of fermented milk.