Expression and inheritance of multiple transgenes in rice plants
Expression and inheritance of multiple transgenes in rice plants
复制标题
DOI：
10.1038/3511
复制
发表时间：
1998-11-01
影响因子：
46.9
通讯作者：
Fauquet, CM
中科院分区：
文献类型：
--
作者：
Chen, LL;Marmey, P;Fauquet, CM
控制多个转基因的整合、遗传和表达的能力是操纵植物生物合成途径和复杂农艺特性的先决条件。用14种不同的pucm质粒共轰击胚性组织，再生了125个独立的转基因水稻植株。85%的R0植株含有2个以上的目标基因，17%含有9个以上的目标基因。含有多个转基因的植株形态正常，63%的植株结实率高。相对于选择标记，多基因共转化效率与质粒混合的比例相关。所有靶基因的整合机会均等，表明编码区的性质对整合效率没有影响。对3个分别含有11个、10个和9个转基因的株系进行了整合和遗传模式分析，直至R3代。多个转基因的整合发生在一个或两个遗传位点上，遗传符合3:1的孟德尔比例。在R2代之前，研究了四个标记基因的共表达。
The ability to control integration, inheritance, and expression of multiple transgenes is a prerequisite for manipulating biosynthetic pathways and complex agronomic characteristics in plants. One hundred and twenty-five independent transgenic rice plants were regenerated after cobombarding embryogenic tissues with a mixture of 14 different pUC-based plasmids. Eighty-five percent of the R0 plants contained more than two, and 17% more than nine, of the target genes. Plants containing multiple transgenes displayed normal morphologies and 63% set viable seed. Multigene cotransformation efficiency was correlated with the ratio in which the plasmids were mixed with respect to the selectable marker. All target genes had an equal chance of integration, indicating that the nature of the coding region had no effect on the efficiency of integration. Three plant lines containing 11, 10, and 9 transgenes, respectively, were analyzed for patterns of integration and inheritance until the R3 generation. Integration of multiple transgenes occurred at either one or two genetic loci, with inheritance conforming to a 3:1 Mendelian ratio. Coexpression of four marker genes was investigated until the R2 generation.