Expression of Transforming Growth Factor β1 and E-Cadherin Proteins in Pulmonary Adenocarcinoma: Its Significance in Tumor Progression
Expression of Transforming Growth Factor β1 and E-Cadherin Proteins in Pulmonary Adenocarcinoma: Its Significance in Tumor Progression
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DOI：
10.4143/crt.2013.45.2.118
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发表时间：
2013-06-01
影响因子：
4.6
通讯作者：
Yoo, Jinyoung
中科院分区：
文献类型：
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作者：
Kim, Chi Hong;Park, Sonya Youngju;Yoo, Jinyoung
目的本研究旨在探讨转化生长因子β1（TGFβ1）和E-钙粘蛋白在肺腺癌肿瘤进展中的意义，并评估其差异表达与形态特征的关系。材料与方法根据国际肺癌研究协会、美国胸科学会和欧洲呼吸学会提出的新分类系统对65例肺腺癌进行重新分类。采用免疫组织化学方法分析 20 例原位腺癌（AIS，以前称为细支气管肺泡癌 [BAC]）、9 例微浸润腺癌（MIA，以前称为 BAC，浸润深度为 5 mm）和 19 例无 BAC 特征的侵袭性腺癌的肿瘤样本中 TGF β 1 和 E-钙粘蛋白的表达。 46% (21/46) 为非侵入性元素，87% (39/45) 为侵入性元素 (p=0.001)。 E-钙粘蛋白在侵袭性成分中的表达频率低于非侵袭性成分（38% vs. 65%，p=0.009）。在非侵入性元件中，TGFβ1 表达与 E-钙粘蛋白表达之间存在负相关性 (p=0.022)。更重要的是，与AIS（5/20，25％）或MIA（2/9，22％）（p = 0.008）相比，LPA的非侵袭性成分中观察到TGFβ1表达频率显着更高（14/17，82％）。结论我们的数据表明TGFβ1和E-钙粘蛋白都参与肺腺癌的肿瘤进展。值得注意的是，TGFβ1 上调先于肿瘤细胞侵袭导致的肺泡破坏。因此，TGF beta 1 可能具有改善肺腺癌诊断和治疗的潜力。
PurposeThis study was conducted in order to investigate the significance of transforming growth factor beta 1 (TGF beta 1) and E-cadherin proteins in tumor progression of lung adenocarcinoma and to evaluate their differential expression in association with morphologic characteristics.Materials and MethodsA total of 65 pulmonary adenocarcinomas were reclassified according to the new classification system proposed by the International Association for the Study of Lung Cancer, American Thoracic Society, and European Respiratory Society. Tumor samples from 20 adenocarcinomas in situ (AIS, formerly bronchioloalveolar carcinoma [BAC]), 9 minimally invasive adenocarcinomas (MIA, formerly BAC with 5 mm invasion), and 19 invasive adenocarcinomas with no BAC features were analyzed by immunohistochemistry for expression of TGF beta 1 and E-cadherin proteins.ResultsTGF beta 1 expression was detected in 46% (21/46) of noninvasive elements and 87% (39/45) of invasive elements (p=0.001). E-Cadherin expression was less frequent in invasive components than in noninvasive components (38% vs. 65%, p=0.009). Negative correlation was identified between TGF beta 1 expression and E-cadherin expression in noninvasive elements (p=0.022). More importantly, significantly higher frequency of TGF beta 1 expression was observed in noninvasive components of LPA (14/17, 82%), compared with those of either AIS (5/20, 25%) or MIA (2/9, 22%) (p=0.008).ConclusionOur data indicate involvement of both TGF beta 1 and E-cadherin proteins in tumor progression of pulmonary adenocarcinoma. It is noteworthy that TGF beta 1 up-regulation precedes alveolar destruction by invasion of tumor cells. TGF beta 1 may thus have the potential to improve lung adenocarcinoma diagnostics and therapeutics.