DENOVO BIOSYNTHESIS OF ARACHIDONIC-ACID AND 5,11,14-EICOSATRIENOIC ACID IN THE CRICKET TELEOGRYLLUS-COMMODUS
DENOVO BIOSYNTHESIS OF ARACHIDONIC-ACID AND 5,11,14-EICOSATRIENOIC ACID IN THE CRICKET TELEOGRYLLUS-COMMODUS
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DOI：
10.1016/0005-2760(88)90333-5
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发表时间：
1988-11-04
期刊：
影响因子：
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通讯作者：
BLOMQUIST, GJ
中科院分区：
文献类型：
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作者：
JURENKA, RA;STANLEYSAMUELSON, DW;BLOMQUIST, GJ
5，11，14-二十碳三烯酸（5，11，14，-20：3）、花生四烯酸（20：4（n - 6））和二十碳二烯酸（20：2（n - 6））的从头生物合成和亚油酸（18：2（n - 6））至20：4（n - 6）的延长/去饱和以及α-亚油酸（18：2（n - 6））至20：4（n - 6）的延长/去饱和。亚麻酸（18：3（n - 3））与二十碳五烯酸（20：5（n - 3））的比例。将[1- 14 C]乙酸钠，[1- 14 C]18：2（n - 6）和[1- 14 C]18：3（n-3）注射到成年雄性蟋蟀中，孵育期后，提取睾丸和剩余组织，并分析从磷脂和三酰甘油馏分中获得的甲酯。每天注射[1- 14 C]乙酸酯5天后，通过AgNO 3-TLC和HPLC纯化睾丸磷脂组分中三烯和四烯脂肪酸的甲酯，并通过GLC、放射性HPLC和臭氧分解产物的放射性GLC进行分析。结果表明，20：2（n - 6），20：4（n-6）和20：3（n - 6）的异构体与双键在5，11，14位的从头生物合成。在向蟋蟀注射放射性标记底物后，通过放射性HPLC分析睾丸磷脂组分中的甲酯，证明了18：2（n - 6）至20：4（n - 6）和18：3（n - 3）至20：5（n - 3）的延长/去饱和。
The de novo biosynthesis of 5,11,14-eicosatrienoic acid (5,11,14,-20:3), arachidonic acid (20:4(n - 6)) and eicosadienoic acid (20:2(n - 6)) and the elongation/desaturation of linoleic acid (18:2(n - 6)) to 20:4(n - 6) and .alpha.-linolenic acid (18:3(n - 3)) to eicosapentaenoic acid (20:5(n - 3)) were demonstrated in adult males of the field cricket Teleogryllus commodus. Sodium [1-14C]acetate, [1-14C]18:2(n - 6) and [1-14C]18:3(n -3) were injected into adult male crickets and after an incubation period, the testes and remaining tissues were extracted and the methyl esters obtained from the phospholipids and triacylglycerol fractions were analyzed. After 5 days of daily injections of [1-14C]acetate, the methyl esters of the triene and tetraene fatty acids from the testicular phospholipid fraction were purified by AgNO3-TLC and HPLC and analyzed by GLC, radio-HPLC, and radio-GLC of ozonolysis products. The results demonstrate the de novo biosynthesis of 20:2(n - 6), 20:4 (n-6) and an isomer of 20:3(n - 6) with double bonds in the 5,11,14 positions. The elongation/desaturation of 18:2(n - 6) to 20:4(n - 6) and 18:3(n - 3) to 20:5(n - 3) was demonstrated by analysis of the methyl esters derived from the testicular phospholipid fraction by radio-HPLC after injecting crickets with radiolabeled substrates.