A novel tumor-related protein, C7orf24, identified by proteome differential display of bladder urothelial carcinoma
A novel tumor-related protein, C7orf24, identified by proteome differential display of bladder urothelial carcinoma
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DOI：
10.1002/prca.200600468
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发表时间：
2007-02-01
影响因子：
2
通讯作者：
Yoshiki, Tatsuhiro
中科院分区：
文献类型：
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作者：
Kageyama, Susumu;Iwaki, Hideaki;Yoshiki, Tatsuhiro
对pH - de范围较窄的膀胱癌进行蛋白质组学分析，发现7号染色体上一个由orf24编码的新蛋白（Oorf24）是癌细胞中高表达的蛋白之一。C7orf24目前作为一种功能未知的假设蛋白注册在蛋白质数据库中。C7orf24在其他动物中的同源物也被登记为推测的蛋白质基因。使用针对C7orf24的单抗进行Western blot分析，证实其在膀胱癌中的表达高于正常组织。其他几种癌细胞系也被发现表达C7orf24。然而，将C7orf24引入Rat-1或NIH3T3细胞并未引起恶性转化。用重组逆转录病毒载体制备了NIH3T3细胞的稳定转染体，并进行了生长速率测定，结果表明，与对照组相比，表达c7orf24的细胞的生长速率更高。然后产生6种小干扰rna (sirna)， C7orf24-siRNA#5对蛋白表达有很强的敲低作用，MTT实验证明其对癌细胞具有显著的抗增殖作用。因此，C7orf24可能在癌细胞增殖中具有重要作用，可能是一种合适的抗癌治疗靶点分子。
Proteome analysis of bladder cancer with narrow-range pH 2-DE has identified a novel protein on chromosome 7 encoded by ORF 24 (Oorf24) as one of the highly expressed proteins in cancer cells. C7orf24 is currently registered in the protein database as a hypothetical protein with unknown function. The homologs of C7orf24 in other animals have also been registered as putative protein genes. Western blot analysis using a mAb against C7orf24 confirmed its higher expression in bladder cancer compared with normal tissue. Several other cancer cell lines were also found to express C7orf24. However, the introduction of C7orf24 into Rat-1 or NIH3T3 cells did not cause malignant transformation. A stable transfectant of NIH3T3 cells with recombinant retrovirus vector was produced for a growth rate assay, and a higher growth rate was observed in C7orf24-expressing cells compared with the controls. Six kinds of small interfering RNAs (siRNAs) were then produced, and C7orf24-siRNA#5 showed a strong knockdown effect on protein expression and significant antiproliferative effects on cancer cell lines were demonstrated by the MTT assay. Therefore, C7orf24 may have an important role in cancer cell proliferation, and may be an appropriate therapeutic target molecule against cancer.