Ultrasmall Graphene Oxide Supported Gold Nanoparticles as Adjuvants Improve Humoral and Cellular Immunity in Mice
Ultrasmall Graphene Oxide Supported Gold Nanoparticles as Adjuvants Improve Humoral and Cellular Immunity in Mice
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DOI：
10.1002/adfm.201401358
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发表时间：
2014-11-26
影响因子：
19
通讯作者：
Zhang, Zhijun
中科院分区：
文献类型：
--
作者：
Cao, Yuhua;Ma, Yufei;Zhang, Zhijun
佐剂在疫苗中起着重要作用。明矾和MF 59是用于人类的两种主要佐剂。然而，这两种疫苗产生对抗癌症和病毒性疾病的疫苗所需的细胞免疫应答的能力有限。希望开发新的和有效的佐剂，目的是改善针对抗原的细胞免疫应答。在这里，超小氧化石墨烯负载的金纳米粒子（usGO-Au）作为一种新的免疫佐剂，以提高免疫反应的可行性和效率进行了探索。usGO-Au由使用usGO还原氯金酸获得，然后通过物理吸附用卵清蛋白（OVA，模型抗原）修饰以构建usGO-Au@OVA。如结果所示，合成的usGO-Au@OVA可以有效地刺激RAW264.7细胞分泌肿瘤坏死因子-（TNF-），其是细胞免疫应答的介质。体内研究表明，usGO-Au@OVA还可以促进稳健的OVA特异性抗体应答、CD 8（+）T细胞增殖和不同细胞因子分泌。结果表明，使用usGO-Au作为佐剂可以刺激针对抗原的有效的体液和细胞免疫应答，这可能有助于更好地理解细胞免疫应答，并促进癌症和病毒疫苗的潜在应用。
Adjuvants play an important role in vaccines. Alum and MF59 are two dominant kinds of adjuvants used in humans. Both of them, however, have limited capacity to generate the cellular immune response required for vaccines against cancers and viral diseases. It is desirable to develop new and efficient adjuvants with the aim of improving the cellular immune response against the antigen. Here, the feasibility and efficiency of ultrasmall graphene oxide supported gold nanoparticles (usGO-Au) as a new immune adjuvant to improve immune responses are explored. usGO-Au is obtained from reduction of chloroauric acid using usGO and then decorated with ovalbumin (OVA, a model antigen) through physical adsorption to construct usGO-Au@OVA. As the results show, the as-synthesized usGO-Au@OVA can efficiently stimulate RAW264.7 cells to secrete tumor necrosis factor- (TNF-), a mediator for cellular immune response. In vivo studies demonstrate that usGO-Au@OVA can also promote robust OVA specific antibody response, CD8(+) T cells proliferation, and different cytokines secretion. The results indicate that using usGO-Au as an adjuvant can stimulate potent humoral and cellular immune responses against antigens, which may promote better understanding of cellular immune response and facilitate potential applications for cancer and viral vaccines.