Human spermidine synthase gene: structure and chromosomal localization.
Human spermidine synthase gene: structure and chromosomal localization.
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人亚精胺合酶基因：结构和染色体定位。
DOI：
10.1089/dna.1991.10.467
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发表时间：
1991
影响因子：
3.1
通讯作者：
J. Jänne
中科院分区：
文献类型：
--
作者：
S. Myöhänen;L. Kauppinen;J. Wahlfors;L. Alhonen;J. Jänne
人亚精胺合酶 (EC 2.5.1.16) 基因是从用从人免疫球蛋白 G (IgG) 骨髓瘤细胞系获得的 DNA 构建的基因组文库中分离出来的。随后的序列分析表明，该基因由从帽位点到推定聚腺苷酸化信号最后一个 A 的 5,818 个核苷酸组成，具有 8 个外显子和 7 个间插序列。该基因的 5' 侧翼区域极其富含 GC，缺乏任何 TATA 框，但包含 CCAAT 共有序列。没有发现 AP-1 结合位点的 cAMP 响应元件的完美共有序列，但该基因包含七个 AP-2 结合位点共有序列。推定的聚腺苷酸化信号是不寻常的 AATACA，而不是 AATAAA。对从人 x 仓鼠体细胞杂种获得的 DNA 进行的聚合酶链式反应分析表明，人亚精胺合酶基因组序列与人 1 号染色体分离。将基因组克隆转染到显示低内源亚精胺合酶活性的中国仓鼠卵巢细胞中，结果表明该基因是瞬时表达的，因此很可能代表功能基因。
The human spermidine synthase (EC 2.5.1.16) gene was isolated from a genomic library constructed with DNA obtained from a human immunoglobulin G (IgG) myeloma cell line. Subsequent sequence analyses revealed that the gene comprised of 5,818 nucleotides from the cap site to the last A of the putative polyadenylation signal with 8 exons and 7 intervening sequences. The 5'-flanking region of the gene was extremely GC rich, lacking any TATA box but containing CCAAT consensus sequences. No perfect consensus sequence for the cAMP-responsive element for the AP-1 binding site was found, yet the gene contained seven AP-2 binding site consensus sequences. The putative polyadenylation signal was an unusual AATACA instead of AATAAA. Polymerase chain reaction analysis with DNA obtained from human x hamster somatic cell hybrids indicated that human spermidine synthase genomic sequences segregate with human chromosome 1. Transfection of the genomic clone into Chinese hamster ovary cells displaying a low endogenous spermidine synthase activity revealed that the gene was transiently expressed and hence in all likelihood represents a functional gene.
编码 S-腺苷甲硫氨酸脱羧酶的基因序列存在于人 6 号染色体和 X 号染色体上，并且在结肠瘤形成中不被扩增。
DOI：
10.1159/000132679
发表时间：
1988
期刊：
Cytogenetics and cell genetics
影响因子：
--
作者：
Radford,DM;Eddy,R;Haley,L;Henry,WM;Pegg,AE;Pajunen,A;Shows,TB
通讯作者：
Shows,TB