Genetic engineering of brewing yeast to reduce the content of ethanol in beer.
Genetic engineering of brewing yeast to reduce the content of ethanol in beer.
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DOI：
10.1111/j.1567-1364.2002.tb00087.x
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发表时间：
2002-05
影响因子：
3.2
通讯作者：
Elke Nevoigt;Rita Pilger;Edeltraud Mast-Gerlach;Ulrike G. Schmidt;Silke Freihammer;M. Eschenbrenner;L. Garbe;U. Stahl
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文献类型：
--
作者：
Elke Nevoigt;Rita Pilger;Edeltraud Mast-Gerlach;Ulrike G. Schmidt;Silke Freihammer;M. Eschenbrenner;L. Garbe;U. Stahl
The GPD1 gene encoding the glycerol-3-phosphate dehydrogenase was overexpressed in an industrial lager brewing yeast (Saccharomyces cerevisiae ssp. carlsbergensis) to reduce the content of ethanol in beer. The amount of glycerol produced by the GPD1-overexpressing yeast in fermentation experiments simulating brewing conditions was increased 5.6 times and ethanol was decreased by 18% when compared to the wild-type. Overexpression of GPD1 does not affect the consumption of wort sugars. Only minor changes in the concentration of higher alcohols, esters and fatty acids could be observed in beer produced by the GPD1-overexpressing brewing yeast. However, the concentrations of several other by-products, particularly acetoin, diacetyl and acetaldehyde, were considerably increased.
