Alterations in the protein synthetic apparatus of Friend erythroleukemia cells infected with vesicular stomatitis virus or herpes simplex virus
Alterations in the protein synthetic apparatus of Friend erythroleukemia cells infected with vesicular stomatitis virus or herpes simplex virus
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水泡性口炎病毒或单纯疱疹病毒感染Friend红白血病细胞蛋白质合成器官的改变
DOI：
10.1128/jvi.25.1.422-426.1978
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发表时间：
1978
影响因子：
5.4
通讯作者：
S. Silverstein
中科院分区：
文献类型：
--
作者：
Y. Nishioka;S. Silverstein
我们比较了单纯疱疹病毒（HSV）和水泡性口炎病毒（VSV）感染对Friend红白血病细胞蛋白质合成装置的影响。先前的研究表明，HSV 感染会迅速停止珠蛋白和其他细胞多肽的合成（Y. Nischioka 和 S. Silverstein，1977，Proc. Natl. Acad. Sci. U.S.A. 74：2370-2374）。与这些发现相反，感染 VSV 的 Friend 红白血病细胞中持续存在珠蛋白合成。使用与多尿苷酸杂交对大量感染的细胞 mRNA 的大小进行物理测量，证明用 VSV 感染后 mRNA 的大小没有可检测到的变化。从感染 HSV 或 VSV 的细胞中提取的细胞质 RNA 与球蛋白互补 DNA 的杂交动力学的比较表明，到 HSV 感染后 4 小时，仅保留约 15% 的球蛋白 mRNA 序列，而 VSV 感染细胞中球蛋白 mRNA 的序列丰度没有明显变化。
We have compared the effects of infection with herpes simplex virus (HSV) and vesicular stomatitis virus (VSV) on the protein synthetic apparatus of Friend erythroleukemia cells. Previous studies demonstrated that infection with HSV rapidly shuts off the synthesis of globin and other cellular polypeptides (Y. Nischioka and S. Silverstein, 1977, Proc. Natl. Acad. Sci. U.S.A. 74: 2370-2374). In contrast to these findings, globin synthesis persists in Friend erythroleukemia cells infected with VSV. Physical measurements of the size of bulk-infected cell mRNA, using hybridization with polyuridylic acid, demonstrated that there was no detectable change in the size of mRNA's after infection with VSV. A comparison of the kinetics of hybridization of cytoplasmic RNA extracted from cells infected with either HSV or VSV with globin complementary DNA revealed that by 4 h postinfection with HSV only about 15% of the globin mRNA sequences remained, whereas there was no discernible change in the sequence abundance of globin mRNA in VSV-infected cells.