Quantification of intra- and extra-cellular thermophilic lipase/esterase production by Thermus sp.
Quantification of intra- and extra-cellular thermophilic lipase/esterase production by Thermus sp.
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DOI：
10.1023/b:bile.0000024092.27943.75
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发表时间：
2004-05-01
影响因子：
2.7
通讯作者：
Longo, MA
中科院分区：
文献类型：
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作者：
Domínguez, A;Sanromán, A;Longo, MA
4株热菌在70℃的液体培养基中培养30 h后产生了溶脂活性。总脂肪酶/酯酶活性最高的是水热菌t -1 (57 U l(-1))，其次是嗜热菌HB27和HB8（分别为33和25 U l(-1)），最后是热菌sp. （16 U l(-1)）。在T. aquaticus t -1和T. thermoophilus HB27中检测到细胞外活性（33和17 U 1(-1)）。所有酶在80时均稳定。随着底物链长度的减少，它们对脂肪酸酯的活性增加（即对己酸对硝基苯酯的水解率比月桂酸酯高6倍）。
Four Thermus strains produced lipolytic activity when grown in liquid medium for 30 h at 70degreesC. The highest total lipase/esterase activity (57 U l(-1)) was in Thermus aquaticus YT-1, followed by Thermus thermophilus HB27 and HB8 (33 and 25 U l(-1), respectively), and finally by Thermus sp. (16 U l(-1)). Extra-cellular activity was detected in T. aquaticus YT-1 and T. thermophilus HB27 (33 and 17 U l(-1)). All enzymes were stable at 80. C over 30 min, and their activity towards fatty acid esters increased as substrate chain-length diminished (i.e. hydrolysis rate was up to 6-fold higher on p-nitrophenyl caproate than on laurate).