Microsatellite repeats in mouse: abundance, distribution and density-猫眼课题宝

DOI：

发表时间：

2006

期刊：

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

X. Tong;F. Dai;Bin Li;Q. Yu;Q. Xia;Cheng Lu

小鼠是动物基因组学中的关键模式生物。获得完整的小鼠染色体序列现在使在全基因组水平上确定SSR频率成为可能。在这项研究中，作者分析了小鼠基因组中不同染色体外显子、内含子和基因间隔区的各种微卫星类型(单核苷酸到六核苷酸重复)的丰度和密度。SSR约占小鼠基因组的2.85%，其中46.2%位于基因间隔区，4.75%位于外显子，49.05%位于内含子。微卫星主要出现在非编码DNA中。微卫星的丰度与染色体或基因组区域的大小呈正相关。然而，它们的总密度与染色体或基因组区域的大小并不密切相关。与其他区域相比，4号染色体外显子区域6种重复类型的丰度均较低。在小鼠基因组中，A、T、AC、AG、AT、AAC、AAG、AGG、AAAC、AAAG、AAAT、AACC、AAAAC、AAAAG、AAAAT、AAAGG、AAAGG、AAAAAC、AAAAAG、AAAAAT、AAAGAG、ACACAT、ACAGAG、ACAGGC、ACATAT是主要的重复类型，而某些五核苷酸重复序列在某些染色体甚至几条染色体上缺失。

The mouse is a pivotal model organism in animal genomics. The availability of complete mouse chromosome sequences now makes possible the determination of frequencies of SSRs at the whole-genome level. In the study, the author analyzed the abundance and density of various microsatellite types (mono-to hexa-nucleotide repeats) in the exonic, intronic and intergenic regions of different chromosomes in the mouse genome. About 2.85 % of the mouse genome was occupied by SSRs, of which 46.2% were detected in the intergenie regions, 4.75% in the exons, and 49.05% in the introns. Microsatellites occurred predominantly in the noncoding DNA. The abundance of mierosatellites was positively correlated with the size of the chromosome or the genomic region. However, their overall density was not closely related to the size of the chromosome or the genomic region. The abundance of all the six repeat types was relatively low in the exonic region of Chromosome 4 as compared with other regions. In the mouse genome, A, T, AC, AG, AT, AAC, AAG, AGG, AAAC, AAAG, AAAT, AACC, AAAAC, AAAAG, AAAAT, AAACC, AAAGG, AAGAG, AAAAAC, AAAAAG, AAAAAT, AAAGAG, ACACAT, ACAGAG, ACAGGC, ACATAT were the predominant repeat types while certain pentanucleotide repeats were absent in a certain chromosome or even in several chromosomes.

Microsatellite repeats in mouse: abundance, distribution and density

文献摘要

被引文献

相似文献