Phospholipid asymmetry during erythrocyte deformation: maintenance of the unit membrane.
Phospholipid asymmetry during erythrocyte deformation: maintenance of the unit membrane.
复制标题
红细胞变形过程中的磷脂不对称：单位膜的维护。
DOI：
10.1016/0005-2736(92)90100-z
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Hebbel,RP
中科院分区：
文献类型：
--
作者：
Sugihara,T;Sugihara,K;Hebbel,RP
为了评估红细胞 (RBC) 膜在变形应力下维持正常磷脂取向的能力，我们检查了经历椭圆形、坦克踩踏变形的红细胞。根据磷脂酶消化的可及性和荧光胺标记确定，正常红细胞能够完全保留其磷脂不对称性，尽管其最大可能变形超过 96%。即使对于 ATP 耗尽或经 vamadate 处理的红细胞，以及由于用叔丁基过氧化氢处理而已经具有不稳定磷脂的红细胞，磷脂取向在变形过程中也不会改变。这些数据表明，在膜的显着变形应力和坦克踩踏运动期间磷脂组织的维持主要归因于膜蛋白的被动稳定作用。这为单位膜的概念提供了额外的证据，该单位膜的特征是脂质和蛋白质之间的密切联系。
To assess the red blood cell (RBC) membrane's ability to maintain normal phospholipid orientation in the face of deforming stress, we examined RBC subjected to elliptical, tank-treading deformation. As determined by accessibility to phospholipase digestion and by labeling with fluorescamine, normal RBC are able to fully preserve their phospholipid asymmetry despite attaining over 96% of their maximal possible deformation. Phospholipid orientation is unchanged during deformation even for RBC that are ATP-depleted or vamadate-treated and for RBC that already have destabilized phospholipids due to treatment witht-butyl hydroperoxide. These data indicate that maintenance of phospholipid organization during marked deforming stress and tank-treading motion of the membrane is ascribable predominantly to the passive stabilizing effect of membrane proteins. This provides additional evidence for the concept of a unit membrane characterized by intimate associations between lipid and protein.