βB2-Crystallin Promotes Axonal Regeneration in the Injured Optic Nerve in Adult Rats
βB2-Crystallin Promotes Axonal Regeneration in the Injured Optic Nerve in Adult Rats
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DOI：
10.3727/096368914x684583
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发表时间：
2015-01-01
影响因子：
3.3
通讯作者：
Thanos, Solon
中科院分区：
文献类型：
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作者：
Boehm, Michael R. R.;Prokosch, Verena;Thanos, Solon
该研究的目的是进一步研究 β B2-晶状体蛋白在体外和体内支持受损视网膜神经节细胞轴突再生的潜力。在视神经挤压 (ONC) 前 5 天或 3 天或同时进行单独晶状体损伤 (LI) 或联合 LI 治疗后，从动物身上获得视网膜外植体，在再生条件下培养 96 小时，并对再生轴突进行量化，并与未治疗的对照进行比较。然后在 ONC 前 5 天重复这些测量，用玻璃体内注射 y-晶状体蛋白和 p-晶状体蛋白代替 LI。最后，在体内粉碎视神经后，研究了眼睛中过表达 332-晶状体蛋白的转染神经祖细胞（β B2-晶状体蛋白-NPC）。借助对病变部位远端和近端的视网膜和视神经进行免疫印迹来监测再生情况，并将其与仅接受磷酸盐缓冲液注射的对照进行比较。 ONC 前 5 天或 3 天进行的 LI 显着促进体外轴突生长 (p
The purpose of the study was to further scrutinize the potential of beta B2-crystallin in supporting regeneration of injured retinal ganglion cell axons both in vitro and in vivo. Retinal explants obtained from animals after treatment either with lens injury (LI) alone or with combined LI 5 days or 3 days before or simultaneously with an optic nerve crush (ONC) were cultured for 96 h under regenerative conditions, and the regenerating axons were quantified and compared with untreated controls. These measurements were then repeated with LI replaced by intravitreal injections of y-crystallin and p-crystallin at 5 days before ONC. Finally, 332-crystallin-overexpressing transfected neural progenitor cells (beta B2-crystallin-NPCs) in the eye were studied after crushing the optic nerve in vivo. Regeneration was monitored with the aid of immunoblotting of the retina and optic nerve both distal and proximal to the lesion site, and this was compared with controls that received injections of phosphate buffer only. LI performed 5 days or 3 days before ONC significantly promoted axonal outgrowth in vitro (p