Homeotic gene Antennapedia mRNA contains 5'-noncoding sequences that confer translational initiation by internal ribosome binding.
Homeotic gene Antennapedia mRNA contains 5'-noncoding sequences that confer translational initiation by internal ribosome binding.
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同源基因 Antennapedia mRNA 包含 5-非编码序列，通过内部核糖体结合赋予翻译起始。
DOI：
10.1101/gad.6.9.1643
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发表时间：
1992
影响因子：
10.5
通讯作者：
Sarnow,P
中科院分区：
文献类型：
--
作者：
Oh,SK;Scott,MP;Sarnow,P
黑腹果蝇（Drosophila melanogaster）的Antp同源异型基因具有两个启动子P1和P2。所得的Antp mRNA含有1512个核苷酸（P1）和1727个核苷酸（P2）的5 '非编码区，分别由外显子A、B、D和E（P1）或外显子C、D和E（P2）组成。多个AUG密码子存在于外显子A、B和C中。我们已经发现，252个核苷酸的外显子D，共同的mRNA从两个转录单位和缺乏AUG密码子，可以介导启动翻译的内部核糖体结合在培养的细胞。果蝇中的许多mRNA含有很长的5 '非编码区，其中有明显未使用的AUG密码子，这表明内部核糖体结合可能是果蝇翻译起始的共同机制，也可能是其调节机制。
The Antennapedia (Antp) homeotic gene of Drosophila melanogaster has two promoters, P1 and P2. The resulting Antp mRNAs contain 1512-nucleotide (P1) and 1727-nucleotide (P2) 5'-noncoding regions, composed of exons A, B, D, and E (P1) or exons C, D, and E (P2), respectively. Multiple AUG codons are present in exons A, B, and C. We have found that 252-nucleotide exon D, common to mRNAs from both transcription units and devoid of AUG codons, can mediate initiation of translation by internal ribosome binding in cultured cells. Many mRNAs in Drosophila contain long 5'-noncoding regions with apparently unused AUG codons, suggesting that internal ribosome binding may be a common mechanism of translational initiation, and possibly its regulation, in Drosophila.