Neurite outgrowth from bipolar and horizontal cells after experimental retinal detachment.
Neurite outgrowth from bipolar and horizontal cells after experimental retinal detachment.
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DOI：
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发表时间：
1998-02
影响因子：
4.4
通讯作者：
G. Lewis;K. Linberg;S. Fisher
中科院分区：
文献类型：
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作者：
G. Lewis;K. Linberg;S. Fisher
目的是研究视网膜中的二阶神经元的水平细胞和杆型双极细胞对实验残留脱离引起的变性的方法。使用免疫细胞化学和电子显微镜分析后，视网膜切片用突触素，Calbindin D和蛋白激酶C（PKC）标记作为突触末端，水平细胞和杆双极细胞的标志物的蛋白质，从脱离后1天开始，在外部丛状的层杂乱无章，并在外部核能受体中发现了突触的光感受器末端。层（ONL）。深入撤回对ONL的光感受器的末端。 Müller细胞或锥形光感受器。为了撤回其突触前靶标，光感受器的合成终端是在受伤后保持合成接触的明显尝试，这表明成人视网膜的可塑性可能对人类患者的视力恢复至关重要。
PURPOSE To study the responses of horizontal cells and rod bipolar cells, the second-order neurons in the retina, to the degeneration induced by experimental retinal detachment. METHODS Retinas from the eyes of domestic cats were examined 1, 3, 7, and 28 days after detachment using immunocytochemical and electron microscopic analyses. Retinal sections were labeled with antibodies to synaptophysin, calbindin D, and protein kinase C (PKC), proteins that serve as markers for synaptic terminals, horizontal cells, and rod bipolar cells, respectively. RESULTS Beginning 1 day after detachment, the outer plexiform layer becomes disorganized and synaptophysin-labeled photoreceptor terminals are detected among the cell bodies of photoreceptors in the outer nuclear layer (ONL). At the same time, horizontal and rod bipolar cell processes grow into the ONL. In some cases, these processes contact photoreceptor terminals that have withdrawn deep into the ONL. Double-labeling experiments with antibodies to glial fibrillary acidic protein (Müller cell labeling) and phosphodiesterase gamma (cone labeling) demonstrate that the calbindin D- and PKC-positive neurite outgrowths are not derived from either Müller cells or cone photoreceptors. CONCLUSIONS Horizontal and rod bipolar cell processes lengthen after retinal detachment, perhaps in response to a withdrawal of their presynaptic targets, the photoreceptor synaptic terminals. This apparent attempt to maintain synaptic contact after injury demonstrates a plasticity in the adult retina that may be of importance for the recovery of vision in human patients.