Disruption of segmental neural crest migration and ephrin expression in Delta-1 null mice
Disruption of segmental neural crest migration and ephrin expression in Delta-1 null mice
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DOI：
10.1006/dbio.2002.0756
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发表时间：
2002-09-01
影响因子：
2.7
通讯作者：
Bronner-Fraser, M
中科院分区：
文献类型：
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作者：
De Bellard, ME;Ching, W;Bronner-Fraser, M
神经嵴细胞迁移节段性通过吻侧的一半，每个躯干体节由于抑制性的影响，肝配蛋白和其他分子存在于尾侧的一半体节。为了研究Notch/Delta信号在建立ephrin节段性分布中的潜在作用，我们研究了Delta-1突变小鼠的神经嵴迁移和ephrin表达。使用Sox-10作为标记，我们注意到，神经嵴细胞移动通过两个吻侧和尾侧的一半的体节的突变体，一致的发现，ephrinB 2水平显着降低尾侧的一半体节。后来，突变胚胎异常融合和/或减少背根和交感神经节，在外周神经胶质细胞显着减少。这些结果表明Delta-1对于神经嵴细胞的适当迁移和分化是必不可少的。有趣的是，Delta-1的缺失导致外周神经节中神经元和神经胶质的减少，这表明突变小鼠中神经节前体库的普遍耗尽。(C)2002 Elsevier Science（美国）。
Neural crest cells migrate segmentally through the rostral half of each trunk somite due to inhibitory influences of ephrins and other molecules present in the caudal-half of somites. To examine the potential role of Notch/Delta signaling in establishing the segmental distribution of ephrins, we examined neural crest migration and ephrin expression in Delta-1 mutant mice. Using Sox-10 as a marker, we noted that neural crest cells moved through both rostral and caudal halves of the somites in mutants, consistent with the finding that ephrinB2 levels are significantly reduced in the caudal-half somites. Later, mutant embryos had aberrantly fused and/or reduced dorsal root and sympathetic ganglia, with a marked diminution in peripheral glia. These results show that Delta-1 is essential for proper migration and differentiation of neural crest cells. interestingly, absence of Delta-1 leads to diminution of both neurons and glia in peripheral ganglia, suggesting a general depletion of the ganglion precursor pool in mutant mice. (C) 2002 Elsevier Science (USA).