The PA subunit is required for efficient nuclear accumulation of the PB1 subunit of the influenza A virus RNA polymerase complex
The PA subunit is required for efficient nuclear accumulation of the PB1 subunit of the influenza A virus RNA polymerase complex
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DOI：
10.1128/jvi.78.17.9144-9153.2004
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发表时间：
2004-09-01
影响因子：
5.4
通讯作者：
Smith, M
中科院分区：
文献类型：
--
作者：
Fodor, E;Smith, M
流感病毒的RNA基因组由细胞核中的病毒RNA聚合酶复合体转录和复制。我们已经产生了绿色荧光蛋白(GFP)标记的聚合酶亚单位来研究聚合酶复合体的组装。结果表明，聚合酶碱性蛋白1(PB1)和聚合酶酸性蛋白(PA)亚基分别分布于细胞质和胞核，而聚合酶碱性蛋白2(PB2)亚基则聚集在胞核。虽然有报道称PB1单独聚集在细胞核中，但我们证明PB1需要PA的共同表达才能有效地积累核。我们的结果支持一种模型，该模型认为PB1和PA作为络合物运输到原子核中。
The RNA genome of influenza virus is transcribed and replicated by the viral RNA polymerase complex in the cell nucleus. We have generated green fluorescent protein (GFP)-tagged polymerase subunits to study the assembly of the polymerase complex. Our results show that individually expressed polymerase basic protein 1 (PB1) and polymerise acidic protein (PA) subunits were distributed in both the cytoplasm and the nucleus, while the polymerase basic protein 2 (PB2) subunit accumulated in the nucleus. Although it has been reported that PB1 alone accumulates in the nucleus, we demonstrate that PB1 requires the coexpression of PA for efficient nuclear accumulation. Our results support a model which proposes that PB1 and PA are transported into the nucleus as a complex.