Nanoparticles from cationic copolymer and DNA that are soluble and stable in common organic solvents
Nanoparticles from cationic copolymer and DNA that are soluble and stable in common organic solvents
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DOI：
10.1021/ja037534v
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发表时间：
2004-01-14
影响因子：
15
通讯作者：
Maiti, S
中科院分区：
文献类型：
--
作者：
Ganguli, M;Jayachandran, KN;Maiti, S
DNA凭借其超强的自组装能力，在设计和制造纳米材料方面的应用已经超越了生物研究。然而，由于DNA在大多数常见有机溶剂中的不溶性，开发用于化学应用的新型DNA基材料可能受到限制。在本通讯中，我们报告了在聚乙二醇（PEG）基阳离子无规共聚物的帮助下，首次证明了DNA可溶于各种非生物溶剂，如乙腈、苯、二甲基亚砜（DMSO）和四氢呋喃。由于阳离子共聚物和阴离子DNA之间形成络合物，形成纳米颗粒。这些纳米粒子有望呈现胶束状结构，其纳米核心是由DNA的磷酸阴离子中和的阳离子单元，周围是PEG片段的外壳。由于PEG可溶于本研究中使用的有机溶剂，纳米颗粒在这些溶剂中是稳定的，使得被包裹的DNA可溶于这些有机溶剂。
DNA by virtue of its superlative ability to self-assemble has found use beyond biological research in the design and fabrication of nanomaterials. However, developing novel DNA-based materials for chemical applications might be restricted due to the insoluble nature of DNA in most common organic solvents. In this Communication, we are reporting the first demonstration of making DNA soluble in a variety of nonbiological solvents such as acetonitrile, benzene, dimethyl sulfoxide (DMSO), and tetrahydrofuran with the help of poly(ethylene glycol) (PEG)-based cationic random copolymers. Because of complex formation between cationic copolymer and anionic DNA, nanoparticles are formed. These nanoparticles are expected to exhibit micelle-like structures with a nanometric core of cationic units neutralized by phosphate anions of DNA, surrounded by a shell of PEG segments. As PEG is soluble in the organic solvents used in this study, nanoparticles are stable in these solvents, making entrapped DNA soluble in these organic solvents.