Expression of livin in renal cell carcinoma and detection of anti-livin autoantibody in patients
Expression of livin in renal cell carcinoma and detection of anti-livin autoantibody in patients
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DOI：
10.1016/j.urology.2007.03.040
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发表时间：
2007-07-01
期刊：
影响因子：
2.1
通讯作者：
Tsukamoto, Taiji
中科院分区：
文献类型：
--
作者：
Kitamura, Hiroshi;Honma, Ichiya;Tsukamoto, Taiji
目的探讨凋亡抑制蛋白家族成员livin在肾细胞癌（renal cell carcinoma，RCC）中的表达及其与预后的关系。采用酶联免疫吸附试验（ELISA）和免疫印迹法（Western blotting）检测患者血清中抗Livin抗体（Abs）。采用Kaplan-Meier法和log-rank检验比较Livin免疫组化阳性组与阴性组、抗Livin Ab阳性组与抗Livin Ab阴性组之间的差异及无复发生存率。结果45例肾细胞癌标本中，26例（57.8%）Livin免疫组化阳性。在RCC患者中检测到抗Livin抗体，其水平显著高于健康志愿者。然而，有没有差异，在疾病特异性生存之间的livin阳性和livin阴性的患者或抗-I livin阳性和抗-livin阴性patients.CONCLUSIONS虽然livin表达可能不提供预测信息，它可能被认为是一种肿瘤抗原，在RCC患者的免疫系统。
OBJECTIVES To assess the expression of livin, a member of the inhibitor of apoptosis protein family, in renal cell carcinoma (RCC) and to determine its prognostic relevance.METHODS Immunohistochemical staining for livin was performed using paraffin-embedded tissues from 45 cases of RCC. Then we assessed anti-livin antibodies (Abs) in patient sera by enzyme-linked immunosorbent assay and Western blotting. Disease-specific survival of patients was assessed, and differences between the immunohistologically livin-positive and livin-negative groups and between the anti-livin Ab-positive and anti-livin Ab-negative groups were compared with recurrence-free survival using the Kaplan-Meier method and log-rank test.RESULTS Of the 45 RCC specimens, 26 (57.8%) showed positive staining of livin immunohistochemically. In the RCC patients, anti-livin antibodies were detected and their levels were significantly higher than those in healthy Volunteers. However, there was no difference in disease-specific survival between the livin-positive and livin-negative patients or between the anti- I livin-positive and anti- livin-negative patients.CONCLUSIONS Although livin expression may not provide predictive information, it may be recognized as a tumor antigen by the immune system in RCC patients.