Quantification of immunohistochemistry - issues concerning methods, utility and semiquantitative assessment II
Quantification of immunohistochemistry - issues concerning methods, utility and semiquantitative assessment II
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DOI：
10.1111/j.1365-2559.2006.02513.x
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发表时间：
2006-10-01
期刊：
影响因子：
6.4
通讯作者：
Levenson, R. M.
中科院分区：
文献类型：
--
作者：
Taylor, C. R.;Levenson, R. M.
免疫组织化学在福尔马林固定石蜡包埋组织上的应用已进入第四个十年。在此期间，该方法已发展成为全球诊断外科病理学实践的主要部分。从一开始，免疫组织化学就被用来提供一系列细胞谱系和组织类型的标志物，特别适用于肿瘤的诊断和分类。在这种模式下，免疫组织化学方法被简单地用作“特殊染色”，其结果由病理学家定性评估，就像任何其他染色一样。最近，注意力已转移到肿瘤细胞中的预后标志物的演示，由分子生物学的出现和许多调节分子的发现，加上相应的特异性抗体的制造驱动。免疫组织化学已经迅速适应了这种新的用途，但在这样做的定量需求已成为最重要的;它不再是足够的“染色”在那里，而是一个问题，“有多少？”'.这篇评论探讨了免疫组化的局限性时，采用半定量模式，并探讨了实现免疫组化的全部潜力的可能性，作为一个真正的定量免疫分析应用于组织切片环境。
Immunohistochemistry is entering its fourth decade of use on formalin-fixed paraffin-embedded tissues. Over this period the method has evolved to become a major part of the practice of diagnostic surgical pathology worldwide. From the beginning immunohistochemistry has been adapted to provide a range of markers of cell lineage and tissue type, with particular application to the diagnosis and classification of tumours. In this modality immunohistochemical methods were employed simply as 'special stains', the results of which were evaluated qualitatively by the pathologist, as for any other stain. More recently, attention has shifted to the demonstration of prognostic markers in tumour cells, driven by the advent of molecular biology and the discovery of numerous regulatory molecules, coupled with manufacture of the corresponding specific antibodies. Immunohistochemistry has rapidly adapted to this new use, but in so doing the demand for quantification has become paramount; it is no longer enough that the 'stain' is there; rather it is a question of 'How much is there?'. This review explores the limitations of immunohistochemistry when employed in a semiquantitative mode, and explores the possibility of fulfilling the full potential of immunohistochemistry, as a true quantitative immunoassay applied in a tissue section environment.