Cross-talk between CD8+ and CD4+ T cells in experimental cutaneous leishmaniasis:: CD8+ T cells are required for optimal IFN-γ production by CD4+ T cells
Cross-talk between CD8+ and CD4+ T cells in experimental cutaneous leishmaniasis:: CD8+ T cells are required for optimal IFN-γ production by CD4+ T cells
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DOI：
10.1111/j.0141-9838.2004.00668.x
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发表时间：
2003-11-01
影响因子：
2.2
通讯作者：
Müller, I
中科院分区：
文献类型：
--
作者：
Herath, S;Kropf, P;Müller, I
虽然CD 8（+）T细胞在利什曼原虫疫苗接种和免疫再感染中的重要性已得到充分证实，但它们在原发性感染中的作用仍存在争议。在本研究中，我们进一步描述了CD 8（+）T细胞在原发性L。严重感染。我们使用了两组L。主要感染BALB/c小鼠：两组均进行免疫操作以愈合，其中一组在整个感染过程中CD 8（+）T细胞被耗尽。我们的研究结果表明，由缺乏CD 8（+）T细胞引起的愈合逆转并没有改变CD 4（+）T细胞的增殖，然而，表达IFN-γ的CD 4（+）T细胞的频率以及这种细胞因子的水平明显降低。这些较低水平的IFN-γ与较高的寄生虫负荷相关。我们的研究结果表明，CD 4（+）T细胞的瞬时消耗允许在CD 4（+）和CD 8（+）T细胞之间建立平衡，并允许CD 8（+）T细胞活化和效应功能的发展。此外，我们的研究结果表明，CD 4（+）和CD 8（+）T细胞之间的相互作用对于宿主防御L。少校
Although the importance of CD8(+) T cells for vaccination and immunity to reinfection with Leishmania parasites is well established, their role in primary infections is disputed. In the present study we further characterized the role of CD8(+) T cells in primary L. major infections. We used two groups of L. major infected BALB/c mice: both groups were immunomanipulated to heal and in one group CD8(+) T cells were depleted throughout the course of infection. Our results show that the reversal of healing caused by the absence of CD8(+) T cells did not alter the proliferation of CD4(+) T cells, however, the frequency of CD4(+) T cells expressing IFN-gamma as well as the levels of this cytokine were clearly reduced. These lower levels of IFN-gamma correlated with a higher parasite load. Our results show that transient depletion of CD4(+) T cells allows the establishment of an equilibrium between CD4(+) and CD8(+) T cells and allows CD8(+) T cell activation and effector functions to develop. In addition, our results suggest that cross-talk between CD4(+) and CD8(+) T cells is crucial for the host defence against L. major.