Dynamic change of microbiota in culture pond based on high-throughput sequencing technique
Dynamic change of microbiota in culture pond based on high-throughput sequencing technique
复制标题
基于高通量测序技术的养殖池微生物群动态变化
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
蔡洪
中科院分区：
文献类型：
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作者：
冯昭中;冯玉晗;王思雨;蔡洪
近年来，水产养殖业是中国高度发展的主要产业之一。水产养殖业每年提供的鱼类食品占我国鱼类食品消费的主要部分。传统的水产养殖技术受制于技术水平等主客观因素，导致水产品质量低劣、价格昂贵，极大地制约了水产养殖业的发展。养殖池塘技术是近年来新兴的一种水产养殖技术，它能有效地滤除水中对鱼类有害的微生物群，进一步有效地提高水产养殖质量，对促进该产业的发展具有重要意义。为此，本文基于高通量测序技术，研究了养殖池塘中微生物区系的动态变化。结论如下：3号循环水养殖系统中微生物区系的丰富度显著高于其他两个循环水养殖系统，且1号循环水养殖系统与2号循环水养殖系统之间存在高度的相似性。就细菌而言，硝化细菌主要是亚硝化单胞菌和硝化螺旋菌。本研究为循环水养殖系统在水产养殖业中的应用提供了参考。
In recent years, aquaculture is one of the highly developed major industries in China. The annual supply.of fish food through aquaculture occupies the main consumption of Chinese people s fish food. The traditional.aquaculture technology is subject to technical level and other subjective or objective factors, resulting in low-quality and.expensive fish product, which greatly restricts the development of aquaculture industry. The culture pond technology,.an emerging aquaculture technology in recent years, can effectively filter out microbiota that are harmful to fish in the.water and further validly improve the quality of aquaculture, which is of great significance to promote the development.of this industry. For this purpose, based on the high-Throughput sequencing technology, this paper studies the.dynamic changes of microbiota in the culture pond. The conclusion is as follows: The richness of microbiota in No.3.recirculating aquaculture system is significantly higher than that of the other two recirculating aquaculture systems, and.there is a high degree of similarity between No.1 recirculating aquaculture system and No.2 recirculating aquaculture.system. As far as bacteria are concerned, the main nitrifying bacteria are nitrosomonas and nitrospira. This study has.provided a reference for the application of the recirculating aquaculture system in the aquaculture industry.