Enzymatic saccharification of pretreated corn stover in a fed-batch membrane bioreactor
Enzymatic saccharification of pretreated corn stover in a fed-batch membrane bioreactor
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DOI：
10.1007/s12155-010-9107-1
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发表时间：
2011-06
影响因子：
3.6
通讯作者：
Mingjia Zhang;R. Su;Qiang Li;W. Qi;Zhimin He
中科院分区：
文献类型：
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作者：
Mingjia Zhang;R. Su;Qiang Li;W. Qi;Zhimin He
在集成膜生物反应器（MBR）中进行玉米秸秆的酶水解，该集成膜生物反应器结合了10 kDa的平板聚砜膜以提高纤维素转化率并减少酶用量。研究了几种预处理方法和半连续式MBR工艺对葡萄糖产量和酶利用率的影响。与传统的间歇式反应器（CBR）相比，由于去除了葡萄糖和纤维二糖抑制剂，MBR中的纤维素转化率提高了5%。流加式膜生物反应器可使纤维素转化率提高15%以上，反应速率也有明显提高。在流加式膜生物反应器中，经氨水浸泡处理的玉米秸秆的酶利用率分别是CBR的1.94倍和1.34倍，经稀硫酸-氢氧化钠处理的玉米秸秆的酶利用率分别是CBR的3.31倍和1.32倍。
Enzymatic hydrolysis of corn stover was performed in an integrated membrane bioreactor (MBR) incorporating a 10 kDa flat sheet polysulfone membrane to increase cellulose conversion and to reduce enzyme dosage. Several pretreatment methods and semi-continuous MBR were examined to investigate their effect on the glucose yield and enzyme utilization efficiency. Compared with conventional batch reactor (CBR), cellulose conversion increased by 5% in a MBR because of the removal of glucose and cellobiose inhibitors. More than 15% increment in cellulose conversion was obtained using fed-MBR, and the reaction rate improved significantly. Enzyme utilization efficiency in a fed-batch MBR were 1.94-fold of CBR and 1.34-fold of fed-CBR for corn stover pretreated by soaking in aqueous ammonia and 3.31-fold of CBR and 1.32-fold of fed-CBR for corn stover pretreated by diluted sulfuric acid–sodium hydroxide.