A radioactive assay allowing the quantitative measurement of cuticular permeability of intact Arabidopsis thaliana leaves
A radioactive assay allowing the quantitative measurement of cuticular permeability of intact Arabidopsis thaliana leaves
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DOI：
10.1007/s00425-011-1381-4
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发表时间：
2011-07-01
期刊：
影响因子：
4.3
通讯作者：
Schreiber, Lukas
中科院分区：
文献类型：
--
作者：
Ballmann, Christina;De Oliveira, Sheron;Schreiber, Lukas
测定了五种不同的c -14标记的化学物质（苯甲酸、双醇、西维因、环氧康唑和4-硝基酚）在拟南芥叶片中的表皮渗透度，并计算了渗透率P （ms(-1)）。因此，对拟南芥叶片的表皮屏障特性进行了定量表征。拟南芥的环氧康唑渗透率为2.79 × 10(-8) ms（-1）。与过去常被用作研究月桂树表皮通透性的模式树种月桂树（Prunus laurocerasus）完整的气孔和气孔叶面所测得的表皮通透性相比，拟李（a.thaliana）的通透性高出48- 66倍。与不同种类柑橘（Citrus aurantium， Hedera helix和P. laurocerasus）离体角质层通透性相比，拟南芥（a.thaliana）通透性高出17- 199倍。通过同时测定三种植物（C-14-epoxiconazole， H. helix， P. laurocerasus）离体角质层（C-14-epoxiconazole）和(H2O)-H-3通透性的共渗实验，发现(H2O)-H-3通透性与epoxiconazole通透性高度相关。这一相关性的回归方程可用于预测拟蓝完整气孔叶片的角质层蒸腾，而用(H2O)-H-3直接测量角质层渗透是不可能的。拟南芥的水透性为4.55 × 10(-8) ms(-1)，比金菖苣、螺旋菖苣和月牙菖苣的水透性高12 ~ 91倍。这表明一年生植物拟南拟楠完整气孔叶片的角质层透水性相对于以往发表的大多数多年生植物的P值较高，处于较高的范围。
Cuticular penetration of five different C-14-labeled chemicals (benzoic acid, bitertanole, carbaryl, epoxiconazole and 4-nitrophenol) into Arabidopsis thaliana leaves was measured and permeances P (ms(-1)) were calculated. Thus, cuticular barrier properties of A. thaliana leaves have been characterized quantitatively. Epoxiconazole permeance of A. thaliana was 2.79 x 10(-8) ms(-1). When compared with cuticular permeances measured with intact stomatous and astomatous leaf sides of Prunus laurocerasus, frequently used in the past as a model species studying cuticular permeability, A. thaliana has a 48- to 66-fold higher permeance. When compared with epoxiconazole permeability of isolated cuticles of different species (Citrus aurantium, Hedera helix and P. laurocerasus) A. thaliana permeability is between 17- to 199-fold higher. Co-permeability experiments, simultaneously measuring C-14-epoxiconazole and (H2O)-H-3 permeability of isolated cuticles of three species (C. aurantium, H. helix and P. laurocerasus) showed that (H2O)-H-3 permeability was highly correlated with epoxiconazole permeability. The regression equation of this correlation can be used predicting cuticular transpiration of intact stomatous leaves of A. thaliana, where a direct measurement of cuticular permeation using (H2O)-H-3 is impossible. Water permeance estimated for A. thaliana was 4.55 x 10(-8) ms(-1), which is between 12- and 91-fold higher than water permeances measured with isolated cuticles of C. aurantium, H. helix and P. laurocerasus. This indicates that cuticular water permeability of the intact stomatous leaves of the annual species A. thaliana is fairly high and in the upper range compared with most P values of perennial species published in the past.