Production of superoxide anion and hydrogen peroxide associated with cell growth of Chattonella antiqua
Production of superoxide anion and hydrogen peroxide associated with cell growth of Chattonella antiqua
复制标题
DOI：
10.3354/ame035057
复制
发表时间：
2004-04-01
影响因子：
1.4
通讯作者：
Kohata, K
中科院分区：
文献类型：
--
作者：
Kim, D;Watanabe, M;Kohata, K
研究了赤潮鞭毛藻Chattonella antiqua(Raphitophyceae)产生活性氧(ROS)的情况。在同步光照条件下(12：12h，L：D期)，在装有1m(3)f/2培养基的NIES(国家环境研究所)培养罐中培养鞭毛。雷氏鞭毛虫在正常生长条件下会形成ROS，如超氧阴离子(O-2(-))和过氧化氢(H2O2)。我们通过化学发光反应证实了C.antiqua根据细胞的生长阶段产生了O-2(-)。O-2(-)产量在指数生长期达到最大值，随后在稳定期下降。而H_2O_2的最大产生量出现在生长稳定期的早期。这些结果表明，安提瓜体内O-2(-)和H_2O_2的产生途径各不相同。每碳含量的O-2(-)和H_2O_2的活性在指数生长期最高，但在细胞生长过程中O-2(-)和H_2O_2的ROS产生模式明显不同。在指数生长期和稳定期对细胞周期进行了两组密集的观察(间隔3h)，在指数生长期，尽管细胞分裂导致细胞密度增加，但O-2(-)和H_2O_2的产生速率在光期增加，而H_2O_2的产生速率在暗期降低，表明这些速率受光合作用的影响。稳定期ROS的产生模式与指数期相似，但生长速率对稳定期的影响小于指数期。
The production of reactive oxygen species (ROS) by the red tide flagellate Chattonella antiqua (Raphidophyceae) was investigated. Flagellates were grown in an NIES (National Institute for Environmental Studies) culture tank containing 1 m(3) of f/2 medium under synchronized light conditions (12:12 h, L:D period). Raphidophycean flagellates are known to form ROS, such as the superoxide anion (O-2(-)) and hydrogen peroxide (H2O2) under normal growth conditions. We confirmed that C. antiqua generated O-2(-) depending on the cell growth phase by chemiluminescence responses. O-2(-) production had a maximum value during the exponential growth phase and subsequently decreased in the stationary phase. However, the maximum production of H2O2 occurred in the early stationary growth phase. These results showed that O-2(-) and H2O2 generation pathways were different from each other in C. antiqua. The highest activity rates of both O-2(-) and H2O2 per carbon content (mug carbon(-1)) were observed during the exponential growth phase, but the pattern of ROS generation was significantly different between O-2(-) and H2O2 during cell growth. Two sets of intensive observations (at 3 h intervals) were conducted for diel phasing of the cell cycle in the exponential and stationary growth phases, In the exponential growth phase, O-2(-) and H2O2 production rates increased during the light period and decreased during the dark period, in spite of the increase in cell density due to cell division, indicating that the rates were affected by photosynthesis. The patterns of ROS production during the stationary growth phase were similar to those in the exponential phase, although the growth rate had less effect during the stationary than in the exponential phase.