Asymptomatic malaria infections and Pfmdr1 mutations in an endemic area of Nigeria
Asymptomatic malaria infections and Pfmdr1 mutations in an endemic area of Nigeria
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DOI：
10.1186/s12936-019-2833-8
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发表时间：
2019-06-27
期刊：
影响因子：
3
通讯作者：
Adebayo, Abiodun H.
中科院分区：
文献类型：
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作者：
Dokunmu, Titilope M.;Adjekukor, Cynthia U.;Adebayo, Abiodun H.
在全球范围内消灭疟疾尚未实现，在许多流行国家仍在持续传播。恶性疟原虫继续对现有抗疟疾药物产生耐药性，给消除疟疾带来巨大问题。本研究评估了寄生虫分离物中无症状感染和耐多药1 （mdr-1）基因突变的频率，这是了解尼日利亚西南部持续高发病率的基础。方法在无症状传播流行病学调查中，对年龄6个月的535例进行筛查。采用富组氨酸蛋白II快速检测试剂盒（RDT）检测健康人的寄生虫流行情况。采用聚合酶链反应（PCR）、限制性内切酶酶切和电泳检测恶性疟原虫mdr-1基因多态性。测序确认多态性。比例比较采用卡方检验，p值为0
BackgroundMalaria eradication globally is yet to be achieved and transmission is sustained in many endemic countries. Plasmodium falciparum continues to develop resistance to currently available anti-malarial drugs, posing great problems for malaria elimination. This study evaluates the frequencies of asymptomatic infection and multidrug resistance-1 (mdr-1) gene mutations in parasite isolates, which form the basis for understanding persistently high incidence in South West, Nigeria.MethodsA total of 535 individuals aged from 6months were screened during the epidemiological survey evaluating asymptomatic transmission. Parasite prevalence was determined by histidine-rich protein II rapid detection kit (RDT) in healthy individuals. Plasmodium falciparum mdr-1 gene mutations were detected by polymerase chain reaction (PCR) followed by restriction enzyme digest and electrophoresis to determine polymorphism in parasite isolates. Sequencing was done to confirm polymorphism. Proportions were compared using Chi-square test at p value