SUCROSE METABOLISM IN NETTED MUSKMELON FRUIT DURING DEVELOPMENT
SUCROSE METABOLISM IN NETTED MUSKMELON FRUIT DURING DEVELOPMENT
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DOI：
10.1104/pp.84.2.386
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发表时间：
1987-06-01
期刊：
影响因子：
7.4
通讯作者：
DUNLAP, JR
中科院分区：
文献类型：
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作者：
LINGLE, SE;DUNLAP, JR
糖含量和成分是衡量网纹甜瓜（Cucumis梅洛L.）变种网状）果实。以“Magnum 45”甜瓜为试材，从授粉后10 d（DAP）开始至果实完全脱落（35 DAP），每隔10 d测定果实糖组成和蔗糖代谢的4种酶。在20和30 DAP之间，外部（绿色）中果皮和内部（橙子）中果皮的糖含量增加。总糖增加的主要部分是由于蔗糖积累。蔗糖相对于葡萄糖和果糖的大幅增加伴随着酸性转化酶活性的急剧下降，这是最高的两个组织在10和20 DAP，并增加蔗糖磷酸合成酶（SPS）和蔗糖合成酶的活性。与橙子组织相比，绿色组织具有较低的总糖比例（以蔗糖计）、较高的转化酶活性和较低的SPS活性。相对蔗糖含量的变化与酶活性的变化高度相关。结果强烈表明，甜瓜果实中发现的蔗糖的比例增加与酸性转化酶活性的下降和SPS活性的增加约10天前全滑。因此，这些酶显然在决定甜瓜果实的糖组成和品质方面起着关键作用。
Sugar content and composition are major criteria used in judging the quality of netted muskmelon (Cucumis melo L. var. reticulatus) fruit. Sugar composition and four enzymes of sucrose metabolism were determined in ''Magnum 45'' muskmelon fruit at 10-day intervals beginning 10 days after pollination (DAP) until full-slip (35 DAP). Sugar content increased in both outer (green) mesocarp and inner (orange) mesocarp between 20 and 30 DAP. The major proportion of toal increase in sugar was attributed to sucrose accumulation. The large increase in sucrose relative to glucose and fructose was accompanied by a dramatic decrease in acid invertase activity, which was highest in both tissues at 10 and 20 DAP, and increases in sucrose phosphate synthase (SPS) and sucrose synthase activities. The green tissue had a lower proportion of total sugar as sucrose, greater invertase activity, and less SPS activity than the orange tissue. Changes in relative sucrose content were highly correlated with changes in enzyme activity. The results strongly suggest that increase in the proportion of sucrose found in melon fruit were associated with a decline in acid invertase activity and an increase in SPS activity approximately 10 days before full-slip. Therefore, these enzymes apparently play a key role in determining sugar composition and the quality of muskmelon fruit.