Influence of 5a-dihydrotestosterone and 17ß-estradiol on human Sertoli cells metabolism
Influence of 5a-dihydrotestosterone and 17ß-estradiol on human Sertoli cells metabolism
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DOI：
10.1111/j.1365-2605.2011.01205.x
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发表时间：
2011-12-01
影响因子：
--
通讯作者：
Socorro, S.
中科院分区：
文献类型：
--
作者：
Oliveira, P. F.;Alves, M. G.;Socorro, S.
支持细胞代谢葡萄糖，将其转化为乳酸，用于发育中的生殖细胞的能量代谢。雄激素和雌激素的代谢作用远远超出了生殖过程。因此，本研究的主要目的是检查性类固醇激素对人支持细胞代谢物分泌/消耗的影响。将富含人支持细胞的原代培养物在限定培养基中维持50小时，并在不存在或存在100 nm 17 β-雌二醇（E2）或100 nm 5 α-二氢睾酮（DHT）的情况下，使用1H-NMR光谱分析测定葡萄糖、丙酮酸盐、乳酸盐和丙氨酸的变化。半定量RT-PCR检测葡萄糖转运蛋白、乳酸脱氢酶和单羧酸转运蛋白的mRNA表达水平。在不存在（对照）或存在E2的情况下培养的细胞在50小时内以相似的速率消耗相同量的葡萄糖。在用DHT处理的第一个15小时期间，葡萄糖消耗和葡萄糖消耗率显著更高。然而，DHT处理的细胞分泌的乳酸盐的量显着低于对照组和E2处理的细胞。这样的减少是伴随着显着减少乳酸脱氢酶A mRNA水平在DHT处理组处理50小时后。最后，丙氨酸生产显着增加E2处理的细胞后25小时的治疗，这表明在这些条件下的细胞较低的氧化还原/较高的氧化状态。这些结果支持性类固醇激素的作用和能量代谢之间的关系的存在，提供了雄激素和雌激素作为人类支持细胞的代谢调节剂的第一次评估。
Sertoli cells metabolize glucose, converting it to lactate that is used by developing germ cells for their energy metabolism. Androgens and oestrogens have metabolic roles that reach far beyond reproductive processes. So, the main purpose of this study was to examine the effect of sex steroid hormones on metabolite secretion/consumption in human Sertoli cells. Human Sertoli cell-enriched primary cultures were maintained in a defined medium for 50 h and glucose, pyruvate, lactate and alanine variations were determined using 1H-NMR spectra analysis, in the absence or presence of 100 nm 17 beta-estradiol (E2) or 100 nm 5a-dihydrotestosterone (DHT). The mRNA expression levels of glucose transporters, lactate dehydrogenase and monocarboxylate transporters were also determined using semi-quantitative RT-PCR. Cells cultured in the absence (control) or presence of E2 consumed the same amounts of glucose at similar rates during the 50 h. During the first 15 h of treatment with DHT, glucose consumption and glucose consumption rate were significantly higher. Nevertheless, DHT-treated cells secreted a significantly lower amount of lactate than control and E2-treated cells. Such a decrease was concomitant with a significant decrease in lactate dehydrogenase A mRNA levels after 50 h treatment in DHT-treated groups. Finally, alanine production was significantly increased in E2-treated cells after 25 h treatment, which indicated a lower redox/higher oxidative state for the cells on those conditions. These results support the existence of a relationship between sex steroid hormones action and energy metabolism, providing the first assessment of androgens and oestrogens as metabolic modulators of human Sertoli cells.