Ultrasound-induced cytolysis of cancer cells is enhanced in the presence of micron-sized alumina particles.
Ultrasound-induced cytolysis of cancer cells is enhanced in the presence of micron-sized alumina particles.
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DOI：
10.1016/j.ultsonch.2007.10.010
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发表时间：
2008-07
影响因子：
8.4
通讯作者：
N. Miyoshi;T. Tuziuti;K. Yasui;Y. Iida;N. Shimizu;P. Riesz;J. Sostaric
中科院分区：
文献类型：
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作者：
N. Miyoshi;T. Tuziuti;K. Yasui;Y. Iida;N. Shimizu;P. Riesz;J. Sostaric
微米尺寸的氧化铝颗粒已被证明可以增强水溶液中声化学自由基的形成，同时提高溶液温度和声学（白）噪声，这些效应可归因于增强的惯性空化作用[T.图齐乌蒂，J. Phys。化学。 109（2005）4869-4872]。在当前的研究中，将相同的超声暴露系统作为模型系统应用于体外癌细胞，以确定氧化铝颗粒对细胞长期存活和细胞死亡主要途径（即凋亡或坏死）的影响。超声处理后孵育6小时后，发现无论是否在有氧化铝颗粒存在的情况下进行暴露，细胞主要通过坏死死亡。氧化铝颗粒本身对细胞无毒，但发现会降低初次暴露后幸存的细胞的长期存活率。这种效果取决于颗粒的大小和浓度。这些结果与相同暴露条件下氧化铝颗粒对 KI 声化学氧化的影响密切相关。 5,5-二甲基吡咯啉 N-氧化物 (DMPO) 的自旋捕获和电子自旋共振光谱表明，在氧化铝颗粒存在下，OH 自由基的声化学形成增加。目前的研究与众所周知的微米级颗粒增强声空化过程的观察结果一致。
Micron-sized alumina particles have been shown to enhance sonochemical free radical formation in aqueous solutions and simultaneously increase the solution temperature and acoustic (white) noise, effects attributable to enhanced inertial cavitation [T. Tuziuti, J. Phys. Chem. A 109 (2005) 4869–4872]. In the current study, the same ultrasound exposure system was applied to in vitro cancer cells as a model system to determine the effect of alumina particles on the long-term survival of cells and on the major pathways of cell death, i.e., either apoptosis or necrosis. Following 6h of incubation after ultrasound treatment, it was found that the cells died mainly through necrosis, irrespective of whether the exposure was conducted in the presence of alumina particles or not. Alumina particles were non-toxic to cells alone, but were found to decrease the long-term survivability of cells that survived the initial exposure. This effect depended on the size and concentration of particles. These results correlated well with the effect of alumina particles on the sonochemical oxidation of KI under the same exposure conditions. Spin-trapping with 5,5-dimethyl-pyroline N-oxide (DMPO) and electron spin resonance spectroscopy indicated that the sonochemical formation of OH radicals increased in the presence of alumina particles. The current study is consistent with the well known observation that micron-sized particles enhance the acoustic cavitation process.