Accuracy Investigation of Phthalate Metabolite Standards
Accuracy Investigation of Phthalate Metabolite Standards
复制标题
DOI：
10.1093/jat/bks016
复制
发表时间：
2012-05-01
影响因子：
2.5
通讯作者：
Thellen, Claude
中科院分区：
文献类型：
--
作者：
Langlois, Eric;LeBlanc, Alain;Thellen, Claude
邻苯二甲酸盐是普遍存在的化合物，其代谢物通常在尿液中测定以用于生物监测研究。根据我们实验室在外部质量评估计划中得出的可疑和无法解释的结果，我们通过与几家供应商的标准品进行比较，调查了我们拥有的所有邻苯二甲酸酯代谢物标准品的准确性。我们的研究结果表明，商业邻苯二甲酸酯代谢物认证的解决方案并不总是准确的，批次之间的差异会显着影响其中几个标准所获得的结果的准确性。这些观察结果表明，从不同批次标准获得的结果的可靠性并不相同，这降低了实验室内和实验室间结果比较的可能性。然而，从不同供应商获得的大多数简洁标准都观察到了准确性协议，这表明该问题的解决方案是可用的。由于所使用的标准，邻苯二甲酸酯代谢物的数据准确性应该成为进行邻苯二甲酸酯代谢物分析的实验室关注的问题。我们的调查结果是从进行邻苯二甲酸酯代谢物分析的实验室将来可以获得准确且可比较的结果的角度提出的。我们的研究结果将有助于提高未来邻苯二甲酸酯代谢物分析的质量，并将影响对过去结果的解释。
Phthalates are ubiquitous compounds whose metabolites are usually determined in urine for biomonitoring studies. Following suspect and unexplained results from our laboratory in an external quality-assessment scheme, we investigated the accuracy of all phthalate metabolite standards in our possession by comparing them with those of several suppliers. Our findings suggest that commercial phthalate metabolite certified solutions are not always accurate and that lot-to-lot discrepancies significantly affect the accuracy of the results obtained with several of these standards. These observations indicate that the reliability of the results obtained from different lots of standards is not equal, which reduces the possibility of intra-laboratory and inter-laboratory comparisons of results. However, agreements of accuracy have been observed for a majority of neat standards obtained from different suppliers, which indicates that a solution to this issue is available. Data accuracy of phthalate metabolites should be of concern for laboratories performing phthalate metabolite analysis because of the standards used. The results of our investigation are presented from the perspective that laboratories performing phthalate metabolite analysis can obtain accurate and comparable results in the future. Our findings will contribute to improving the quality of future phthalate metabolite analyses and will affect the interpretation of past results.