Label-free turn-on fluorescent detection of melamine based on the anti-quenching ability of Hg2+ to gold nanoclusters
Label-free turn-on fluorescent detection of melamine based on the anti-quenching ability of Hg2+ to gold nanoclusters
复制标题
DOI：
10.1016/j.bios.2013.09.034
复制
发表时间：
2014-03-15
影响因子：
12.6
通讯作者：
Li, Zhuang
中科院分区：
文献类型：
--
作者：
Dai, Haichao;Shi, Yan;Li, Zhuang
在这项工作中，我们提出了一种简便，环保和成本效益的三聚氰胺与BSA稳定的金纳米团簇（AuNCs）作为荧光阅读器的分析。三聚氰胺是一种多氮杂环化合物，它与Hg ~（2+）有较好的配位作用。这一性质通过减弱Hg 2+与Au+之间的亲金属作用而使Hg 2+对AuNCs具有抗猝灭能力。该方法的检出限为0.15 μ M，比美国食品药品监督管理局（FDA）对三聚氰胺安全限量20 μ M的估计值低约130倍。此外，使用该传感系统对多个掺有三聚氰胺的真实的样品（包括原料奶和奶粉）进行了分析，并获得了良好的回收率。这种基于金纳米团簇的荧光方法可以应用于真实的样品中三聚氰胺的高灵敏检测。(C)2013爱思唯尔有限公司版权所有。
In this work, we proposed a facile, environmentally friendly and cost-effective assay for melamine with BSA-stabilized gold nanoclusters (AuNCs) as a fluorescence reader. Melamine, which has a multi-nitrogen heterocyclic ring, is prone to coordinate with Hg2+. This property causes the anti-quenching ability of Hg2+ to AuNCs through decreasing the metallophilic interaction between Hg2+ and Au+. By this method, detection limit down to 0.15 mu M is obtained, which is approximately 130 times lower than that of the US food and Drug Administration estimated melamine safety limit of 20 mu M. Furthermore, several real samples spiked with melamine, including raw milk and milk powder, are analyzed using the sensing system with excellent recoveries. This gold-nanocluster-based fluorescent method could find applications in highly sensitive detection of melamine in real samples. (C) 2013 Elsevier B.V. All rights reserved.